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Ezrin Antibody (YA3808)

(Synonyms: CVL; CVIL; VIL2; HEL-S-105)
Customer Review

Based on 1 Customer Validation

Ezrin Antibody (YA3808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Ezrin Antibody (YA3808) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100-1:400 1:200-1:400 1:10000

Product Details

Description

Ezrin Antibody (YA3808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Immunogen

Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ezrin Antibody (YA3808)
    Western blot analysis of extracts from HCT116(lane 2(20ug) , Hela(lane 3(20ug) and C6(lane 4(20ug),NIH/3T3(lane 5(20ug) using Ezrin Antibody (HY-P84111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    Flow Cytometry analysis of Hela cells labelling Ezrin (red) with Ezrin Antibody (anti-Ezrin) (HY-P84111). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    Immunocytochemistry analysis of Hela cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    Immunocytochemistry analysis of A549 cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Ezrin is a Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis

  • Subcellular Localization

    Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cell cortex; Cytoplasm, cytoskeleton; Cell projection, microvillus

  • Expression


    Tissue_specificity:It is expressed in the cerebral cortex, basal ganglia, hippocampus, pituitary gland, and optic nerve. Expression is weaker in the brainstem and diencephalon. Expression is stronger in the frontal gray matter than in white matter (protein level) . It is a component of the microvilli of intestinal epithelial cells. It is preferentially expressed in astrocytes of the hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Expression was not detected in neurons in most of the tissues studied.

  • Subunit

    Monomer. Homodimer (PubMed:27364155). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, NHERF1 and TMEM8B (PubMed:12651155, PubMed:15498789, PubMed:17616675, PubMed:17881735, PubMed:19555689, PubMed:9314537).

  • SwissProt ID

    P15311

  • Gene ID
    7430 [NCBI]
  • Synonyms

    CVL; CVIL; VIL2; HEL-S-105

Ezrin Antibody (YA3808) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , Hela(lane 3(20ug) and C6(lane 4(20ug),NIH/3T3(lane 5(20ug) using Ezrin Antibody (HY-P84111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow Cytometry analysis of Hela cells labelling Ezrin (red) with Ezrin Antibody (anti-Ezrin) (HY-P84111). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ezrin Antibody (YA3808) Product Image
    1/16
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Ezrin Antibody (YA3808) Product Image
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , Hela(lane 3(20ug) and C6(lane 4(20ug),NIH/3T3(lane 5(20ug) using Ezrin Antibody (HY-P84111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    1/1
    MedChemExpress Validation
     Flow Cytometry analysis of Hela cells labelling Ezrin (red) with Ezrin Antibody (anti-Ezrin) (HY-P84111). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ezrin Antibody (YA3808)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Experimental Validation Results for Ezrin Antibody (YA3808)
  • Ezrin Antibody (YA3808) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116(lane 2(20ug) , Hela(lane 3(20ug) and C6(lane 4(20ug),NIH/3T3(lane 5(20ug) using Ezrin Antibody (HY-P84111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ezrin Antibody (YA3808) Product Image
    MedChemExpress Validation

    Flow Cytometry analysis of Hela cells labelling Ezrin (red) with Ezrin Antibody (anti-Ezrin) (HY-P84111). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Ezrin Antibody (YA3808) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ezrin Antibody (YA3808) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Ezrin with Ezrin Antibody (HY-P84111) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ezrin Antibody (HY-P84111) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ezrin Antibody (YA3808) Product Image WB
  • Ezrin Antibody (YA3808) Product Image FC
  • Ezrin Antibody (YA3808) Product Image ICC
  • Ezrin Antibody (YA3808) Product Image ICC