BID Antibody (YA4746)

Cat. No.: HY-P85054: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

BID Antibody (YA4746) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BID.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

BID Antibody (YA4746) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BID.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 22 kDa;

Species Reactivity

Human

SwissProt ID

P55957

Gene ID

637 [NCBI]

Immunogen

Purified recombinant fragment of human BID expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity); Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622); Induces ICE-like proteases and apoptosis; Induces ICE-like proteases and apoptosis; Does not induce apoptosis; Induces ICE-like proteases and apoptosis

Subcellular Localization：Cytoplasm; Mitochondrion membrane; Mitochondrion outer membrane; Mitochondrion membrane; Mitochondrion membrane; Cytoplasm; Cytoplasm; Mitochondrion membrane

Expression：
Tissue_specificity:It is expressed in the spleen, pancreas, and placenta (protein level) ; it is expressed in the lungs, pancreas, and spleen (protein level) ; it is expressed in the lungs and pancreas (protein level) .

Isoforms & Post-Translational Modification：P55957 has 4 isomers: P55957-1: 21995 Da (predicted); P55957-2: 26836 Da (predicted); P55957-3: 14620 Da (predicted); P55957-4: 11263 Da (predicted).
TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622);Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation

Subunit：Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2 (By similarity). Interacts with PLEKHN1 (PubMed:29531808)

Synonyms

BID; BH3-interacting domain death agonist; p22 BID; BID

Documentación

User Guide for Antibodies (1077 KB)