DGCR8 Antibody (YA2680)(PBS only)
(Synonyms: DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha)DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 86 kDa
Recombinant protein of human DGCR8 aa424-773.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using DGCR8 (HY-P82935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
DGCR8 component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal. Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Nucleus; Nucleus, nucleolus
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Expression
Tissue_specificity:Ubiquitously expressed -
Isoforms & Post-Translational Modification
Q8WYQ5 has 3 isomers: Q8WYQ5-1: 86045 Da (predicted); Q8WYQ5-2: 32831 Da (predicted); Q8WYQ5-3: 82750 Da (predicted).
Phosphorylated at Ser-677 by ATM upon radiation, which is crucial for its stability;Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization -
Subunit
Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization (PubMed:17159994).
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SwissProt ID
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Synonyms
DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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