DGCR8 Antibody (YA2680)(PBS only)

Cat. No.: HY-P82935A: Data Sheet User Guide for Antibodies
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DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.

Host

Rabbit

Molecular Weight

Predicted band size: 86 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8WYQ5

Gene ID

54487 [NCBI]

Immunogen

Recombinant protein of human DGCR8 aa424-773.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks (PubMed:34188037)

Subcellular Localization：Nucleus; Nucleus, nucleolus

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：Q8WYQ5 has 3 isomers: Q8WYQ5-1: 86045 Da (predicted); Q8WYQ5-2: 32831 Da (predicted); Q8WYQ5-3: 82750 Da (predicted).
Phosphorylated at Ser-677 by ATM upon radiation, which is crucial for its stability;Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization

Subunit：Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization (PubMed:17159994).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha

Documentation

User Guide for Antibodies (1077 KB)