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DGCR8 Antibody (YA2680)

(Synonyms: DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha)
Customer Review

Based on 1 Customer Validation

DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • DGCR8 Antibody (YA2680) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

DGCR8 Antibody (YA2680) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGCR8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human DGCR8 aa424-773.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105054

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using DGCR8 (HY-P82935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    DGCR8 component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal. Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Nucleus; Nucleus, nucleolus

  • Expression


    Tissue_specificity:Ubiquitously expressed

  • Isoforms & Post-Translational Modification

    Q8WYQ5 has 3 isomers: Q8WYQ5-1: 86045 Da (predicted); Q8WYQ5-2: 32831 Da (predicted); Q8WYQ5-3: 82750 Da (predicted).
    Phosphorylated at Ser-677 by ATM upon radiation, which is crucial for its stability;Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization

  • Subunit

    Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization (PubMed:17159994).

  • SwissProt ID

    Q8WYQ5

  • Gene ID
    54487 [NCBI]
  • Synonyms

    DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha

  • Research Field

    Epigenetics and Nuclear Signaling

DGCR8 Antibody (YA2680) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using DGCR8 (HY-P82935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DGCR8 Antibody (YA2680) Product Image
    1/16
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • DGCR8 Antibody (YA2680) Product Image
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using DGCR8 (HY-P82935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • Experimental Validation Results for DGCR8 Antibody (YA2680)
  • DGCR8 Antibody (YA2680) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using DGCR8 (HY-P82935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • DGCR8 Antibody (YA2680) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DGCR8 Antibody (YA2680) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling DGCR8 with DGCR8 antibody (HY-P82935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with DGCR8 antibody (HY-P82935) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DGCR8 Antibody (YA2680) Product Image WB
  • DGCR8 Antibody (YA2680) Product Image ICC
  • DGCR8 Antibody (YA2680) Product Image ICC