RNF114 Antibody (YA6918)

Cat. No.: HY-P87230: Data Sheet User Guide for Antibodies
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RNF114 Antibody (YA6918) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNF114.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

RNF114 Antibody (YA6918) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNF114.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size：26 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y508

Immunogen

Recombinant protein within human RNF114 aa 1-228 / 228.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates (PubMed:23645206, PubMed:25165885). In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity (PubMed:25165885, PubMed:28708287). Acts as a negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3 (PubMed:25165885). May promote the ubiquitination of TRAF6 as well (PubMed:28708287). Also acts as a negative regulator of T-cell activation (PubMed:25165885). Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation (PubMed:25165885). Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C (PubMed:23645206). This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis

Isoforms & Post-Translational Modification：Q9Y508 has two isomers: Q9Y508-1: 25694 Da (predicted); Q9Y508-2: 21004 Da (predicted).
Autoubiquitinated

Subunit：Interacts with XAF1, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling

Synonyms

1110008J21Rik antibody

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]