Mesothelin Antibody (YA4236)(PBS only)

Cat. No.: HY-P84539A: Data Sheet User Guide for Antibodies
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Mesothelin Antibody (YA4236) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Mesothelin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Mesothelin Antibody (YA4236) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Mesothelin.

Host

Mouse

Molecular Weight

Predicted band size: 69 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

Q13421

Gene ID

10232 [NCBI]

Immunogen

Purified recombinant fragment of human MSLN (AA: 37-286) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Membrane-anchored forms may play a role in cellular adhesion; Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Golgi apparatus; Secreted; Secreted

Expression：
Tissue_specificity:It is expressed in the lungs. It is expressed at low levels in the heart, placenta, and kidneys. It is expressed in mesothelial cells. It is highly expressed (protein level) in mesothelioma, ovarian cancer, and certain squamous cell carcinomas.

Isoforms & Post-Translational Modification：Q13421 has 4 isomers: Q13421-1: 68986 Da (predicted); Q13421-3: 68070 Da (predicted); Q13421-2: 71492 Da (predicted); Q13421-4: 67940 Da (predicted).
Both MPF and the cleaved form of mesothelin are N-glycosylated;Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin

Subunit：Interacts with MUC16

Synonyms

MPF; SMRP

Documentation

User Guide for Antibodies (1077 KB)