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SMARCB1 Antibody (YA5649)

(Synonyms: BAF47; BRG1-associated factor 47; hSNF5; INI1; Integrase interactor 1 protein; Malignant rhabdoid tumor suppressor; RDT; RTPS1; Sfh1p; SMARCB1; SNF5 homolog; SNF5_HUMAN; SNF5L1; Snr1; Sucrose nonfermenting yeast homolog like 1; SWI/SNF complex component SNF5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5; TYE4)

SMARCB1 Antibody (YA5649) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SMARCB1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse,

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • SMARCB1 Antibody (YA5649) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-500 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

SMARCB1 Antibody (YA5649) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SMARCB1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse,
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Immunogen

Synthesized peptide derived from human INI-1 AA range: 300-385

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    SMARCB1 encodes a core SWI/SNF/BAF chromatin-remodeling subunit that supports chromatin access and transcriptional regulation[1]. Mechanistically, SMARCB1 enables BAF-mediated enhancer activation and helps activate bivalent promoters through opposition to Polycomb repression[2]. In malignant rhabdoid tumor models, biallelic SMARCB1 loss defines disease biology and rewires patient-specific distal enhancers linked to MYC expression[3][4]. Compared with related BAF assemblies, SMARCB1-containing complexes preferentially occupy enhancers, whereas BRD9-containing SMARCB1-lacking complexes localize at promoters and enhancers and create a BRD9 vulnerability after SMARCB1 loss[5]. SMARCB1 has two splice isoforms generated by alternative splicing within exon 2, producing 385- and 376-amino-acid proteins[6]. For experimental applications, SMARCB1-deficient models support studies of enhancer regulation, Polycomb antagonism, MYC-driven oncogenic transcription, and BRD9 dependency[2][3][5].- SMARCB1 links BAF chromatin remodeling to enhancer activation and Polycomb-opposed promoter regulation. - SMARCB1-deficient tumor models enable mechanistic studies of MYC enhancers and BRD9 vulnerability.

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q12824 has 2 isomers: Q12824-1: 44141 Da (predicted); Q12824-2: 43158 Da (predicted).

  • Subunit

    Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B) and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific (Probable). Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3 (PubMed:18765789, PubMed:8895581). Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin (By similarity). Component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin (PubMed:26601204). Binds to double-stranded DNA. Interacts with CEBPB (when not methylated) (PubMed:20111005). Interacts with PIH1D1 (PubMed:22368283). Interacts with MYK and MAEL (PubMed:10319872). Interacts with PPP1R15A (PubMed:10490642, PubMed:12016208). Interacts with DPF2 (PubMed:20460684). Interacts with YWHAZ (PubMed:16959763). Interacts with ERCC6 (PubMed:24874740). Interacts with FOS, FOSB isoform 1 and 2, FOSL1 and FOSL2 (By similarity)

  • SwissProt ID

    Q12824

  • Gene ID
    6598 [NCBI]
  • Synonyms

    BAF47; BRG1-associated factor 47; hSNF5; INI1; Integrase interactor 1 protein; Malignant rhabdoid tumor suppressor; RDT; RTPS1; Sfh1p; SMARCB1; SNF5 homolog; SNF5_HUMAN; SNF5L1; Snr1; Sucrose nonfermenting yeast homolog like 1; SWI/SNF complex component SNF5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5; TYE4

References

[1]. SMARCB1 gene information from NCBI.

[2]. Nakayama RT, et al. SMARCB1 is required for widespread BAF complex-mediated activation of enhancers and bivalent promoters. Nat Genet. 2017 Nov;49(11):1613-1623. [Content Brief]

[3]. Versteege I, et al. Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer. Nature. 1998 Jul 9;394(6689):203-6. [Content Brief]

[4]. Liu NQ, et al. SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors. Nat Commun. 2023 Dec 1;14(1):7762. [Content Brief]

[5]. Wang X, et al. BRD9 defines a SWI/SNF sub-complex and constitutes a specific vulnerability in malignant rhabdoid tumors. Nat Commun. 2019;10(1):1881. [Content Brief]

[6]. Bruder CE, et al. The mouse ortholog of the human SMARCB1 gene encodes two splice forms. Biochem Biophys Res Commun. 1999 Apr 21;257(3):886-90. [Content Brief]

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SMARCB1 Antibody (YA5649) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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