2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. CHD3 Antibody (YA944)

CHD3 Antibody (YA944) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHD3.

For research use only. We do not sell to patients.

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50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

  • References

Description

CHD3 Antibody (YA944) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHD3.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 227 kDa;
Observed band size: 260 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed:28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666, PubMed:30397230, PubMed:9804427). Involved in transcriptional repression as part of the NuRD complex (PubMed:27068747). Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity (PubMed:17626165)
Subcellular Localization：Nucleus, PML body; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Expression：
Tissue_specificity:Broad expression
Isoforms & Post-Translational Modification：Q12873 has 3 isomers: Q12873-1: 226592 Da (predicted); Q12873-2: 222861 Da (predicted); Q12873-3: 233037 Da (predicted).
Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression
Subunit：Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:16428440, PubMed:27068747, PubMed:28977666, PubMed:33283408, PubMed:9804427). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:28977666, PubMed:33283408). Interacts with CBX1 (PubMed:28977666). Interacts with CBX3 (PubMed:28977666). Interacts with CBX5 (PubMed:28977666). Interacts (via its C-terminal) with HABP4 (PubMed:12505151). Interacts with HDAC1 (PubMed:28977666). Interacts with MTA1 (PubMed:28977666). Interacts with PCNT; the interaction regulates centrosome integrity (PubMed:17626165). Interacts with RBBP7 (PubMed:28977666). Interacts with SERBP1 (PubMed:12505151). Interacts with TRIM28 (PubMed:11230151). Interacts with ZBED1/hDREF (PubMed:27068747)
Database

Entrez Gene: 1107 Human ; 216848 Mouse ; 303241 Rat

SwissProt: Q12873 Human ;

OMIM: 618205 Human

Research Field

Immunology
Synonyms
CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
Documentation
User Guide for Antibodies (1077 KB)
References

CHD3 Antibody (YA944) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CHD3 Antibody (YA944)
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