CHD3 Antibody (YA944)
(Synonyms: CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH)CHD3 Antibody (YA944) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHD3.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
CHD3 Antibody (YA944) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHD3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 260 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 227 kDa
Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CHD3 is an ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity[1][2][3][4][5][6].
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Subcellular Localization
Nucleus, PML body; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_specificity:Broad expression -
Isoforms & Post-Translational Modification
Q12873 has 3 isomers: Q12873-1: 226592 Da (predicted); Q12873-2: 222861 Da (predicted); Q12873-3: 233037 Da (predicted).
Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression -
Subunit
Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:16428440, PubMed:27068747, PubMed:28977666, PubMed:33283408, PubMed:9804427). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:28977666, PubMed:33283408). Interacts with CBX1 (PubMed:28977666). Interacts with CBX3 (PubMed:28977666). Interacts with CBX5 (PubMed:28977666). Interacts (via its C-terminal) with HABP4 (PubMed:12505151). Interacts with HDAC1 (PubMed:28977666). Interacts with MTA1 (PubMed:28977666). Interacts with PCNT; the interaction regulates centrosome integrity (PubMed:17626165). Interacts with RBBP7 (PubMed:28977666). Interacts with SERBP1 (PubMed:12505151). Interacts with TRIM28 (PubMed:11230151). Interacts with ZBED1/hDREF (PubMed:27068747)
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SwissProt ID
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Synonyms
CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
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Research Field
Immunology
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