2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Angiopoietin 2 Antibody (YA1598)

Angiopoietin 2 Antibody (YA1598)

Art. -Nr.: HY-P81853
COA
SDS
User Guide for Antibodies Technical Support

Angiopoietin 2 Antibody (YA1598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Angiopoietin 2.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe Preis Verfügbarkeit Menge
Kostenlose Probe   Apply now  
10 μL Auf Lager
50 μL Auf Lager
100 μL Auf Lager
250 μL   Angebot einholen  
or Massenanfrage

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Beschreibung

Beschreibung

Angiopoietin 2 Antibody (YA1598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Angiopoietin 2.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molekulargewicht
Predicted band size: 57 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Angiopoietin 2 aa365-410.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
ALL WB IHC-P

  • Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using Angiopoietin 2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Angiopoietin 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Angiopoietin 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Involved in the regulation of lymphangiogenesis (PubMed:32908006)
Subcellular Localization：Secreted
Isoforms & Post-Translational Modification：O15123 has 3 isomers: O15123-1: 56919 Da (predicted); O15123-2: 50958 Da (predicted); O15123-3: 56848 Da (predicted).
Subunit：Interacts with TEK/TIE2, competing for the same binding site as ANGPT1 (PubMed:12427764, PubMed:15284220, PubMed:19223473, PubMed:32908006, PubMed:9204896). Interacts with ITGA5 (PubMed:32908006). Interacts with SVEP1/polydom (By similarity). Interacts with THBD; this interaction significantly inhibits the generation of activated PC and TAFIa/CPB2 by the thrombin/thrombomodulin complex (PubMed:29323190)
RRID
Database

Entrez Gene: 285 Human ; 11601 Mouse ; 89805 Rat

SwissProt: O15123 Human ; O35608 Mouse ; O35462 Rat

OMIM: 619369 Human

Research Field

Cardiovascular
Synonyms
Angiopoietin-2 (ANG-2)
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Angiopoietin 2 Antibody (YA1598) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Ihre kürzlich angesehenen Produkte:

Online-Anfrage

Your information is safe with us. * Required Fields.

Produktname

  

Requested Quantity *

Name des Antragstellers *

 

Anrede

E-Mail-Addresse *

 

Telefonnummer *

Department

 

Name der Organisation *

City

Bundesland

Country or Region *

      

Bemerkungen

Massenanfrage

Inquiry Information

Produktname:
Angiopoietin 2 Antibody (YA1598)
Art. -Nr.:
HY-P81853
Menge:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Produktname

  

Lot #

Name des Antragstellers *

 

Country or Region *

E-Mail-Addresse *

     

Telefonnummer *

 

Name der Organisation *

Bemerkungen

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.