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c-Fos Antibody

(Synonyms: FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7)
4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

c-Fos Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to c-Fos.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • c-Fos Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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4 Publications Citing Use of MCE c-Fos Antibody (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:10000

Product Details

Description

c-Fos Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to c-Fos.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Fos.AA range:331-380

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102634

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for c-Fos Antibody
    Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg) using c-Fos Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for c-Fos Antibody
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using c-Fos Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Fos Antibody
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for c-Fos Antibody
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    c-Fos is an immediate-early gene product and transcription factor that functions as a central mediator of stimulus-dependent gene expression, linking transient extracellular signals to long-term cellular responses through regulation of downstream target genes[1]. Mechanistically, c-Fos belongs to the Fos family and forms the activator protein-1 (AP-1) transcription factor complex through dimerization with Jun family proteins, thereby regulating genes involved in cellular differentiation, proliferation, development, plasticity, and survival[4][3][2]. c-Fos expression is rapidly induced by neuronal activity, calcium- and cAMP-dependent signaling, and other environmental stimuli, making it a widely used molecular marker of cellular activation and stimulus-transcription coupling in experimental systems[1][3]. In disease and experimental models, c-Fos has been extensively employed to map activated neuronal populations during seizures, learning, memory formation, and behavioral responses, providing a framework for investigating neural circuit function and activity-dependent transcriptional programs[1][3]. Compared with related Fos family members, including FosB, Fra-1 (FOSL1), and Fra-2 (FOSL2), c-Fos contains a C-terminal transactivation domain and exhibits distinctive transcriptional regulatory properties within AP-1 complexes[4]. For experimental applications, c-Fos is primarily used as an activity-dependent biomarker rather than a direct pharmacological target, enabling quantitative assessment of cellular activation, neural network recruitment, and stimulus-responsive signaling pathways in neuroscience and molecular biology research[3][5].

  • Subcellular Localization

    Nucleus; Endoplasmic reticulum; Cytoplasm, cytosol

  • Isoforms & Post-Translational Modification

    P01100 has 3 isomers: P01100-1: 40695 Da (predicted); P01100-2: 28986 Da (predicted); P01100-3: 36301 Da (predicted).
    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity);Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232;In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis

  • Subunit

    Heterodimer; with JUN (By similarity). Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site (PubMed:9732876). Interacts with SMAD3; the interaction is weak even on TGF-beta activation (PubMed:9732876). Interacts with MAFB (By similarity). Interacts with TSC22D3 (via N-terminus); this interaction inhibits the binding of active AP1 to its target DNA (By similarity). Interacts with CDS1 and PI4K2A (By similarity). Interacts (via bZIP domain and leucine-zipper region) with the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF) subunits SMARCB1, SMARCC2 and SMARCD1 (By similarity). Interacts (via bZIP domain and leucine-zipper region) with ARID1A (By similarity)

  • SwissProt ID

    P01100

  • Gene ID
    2353 [NCBI]
  • Synonyms

    FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7

  • Research Field

    Neuroscience

[1]. Gallo FT, et al. Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Front Behav Neurosci. 2018;12:79.

[2]. Curran T, et al. Fos: an immediate-early transcription factor in neurons. J Neurobiol. 1995 Mar;26(3):403-12. [Content Brief]

[3]. Lara Aparicio SY, et al. Current Opinion on the Use of c-Fos in Neuroscience. NeuroSci. 2022;3(4):687-702.

[4]. Srivastava SK, et al. Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol. Gene. 2014 Sep 10;548(1):142-8. [Content Brief]

[5]. Cruz-Mendoza F, et al. Immediate early gene c-fos in the brain: focus on glial cells. Brain Sci. 2022;12(6):687. [Content Brief]

[6]. Lara Aparicio SY, et al. Current opinion on the use of c-Fos in neuroscience. NeuroSci. 2022;3(4):687-702. [Content Brief]

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c-Fos Antibody Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg) using c-Fos Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using c-Fos Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    c-Fos Antibody purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 31;23(1):142. [Abstract]

    Customer Validation
     Representative Western blot images showing Nfatc1, Ctsk, Fos, Mmp9, and Actin in BMM lysates. Primary antibodies used were anti-Nfatc1 (1:1,000), anti-Fos (c-Fos Antibody, 1:1,000; overnight at 4 °C, HY-P80616), anti-Ctsk (1:1,000, HY-P81163) and anti-MMP9 (1:1,000).

  • c-Fos Antibody Product Image
    1/16
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • c-Fos Antibody Product Image
  • Experimental Validation Results for c-Fos Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg) using c-Fos Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for c-Fos Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using c-Fos Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Fos Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for c-Fos Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody
  • Experimental Validation Results for c-Fos Antibody

  • c-Fos Antibody purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 31;23(1):142. [Abstract]

    Representative Western blot images showing Nfatc1, Ctsk, Fos, Mmp9, and Actin in BMM lysates. Primary antibodies used were anti-Nfatc1 (1:1,000), anti-Fos (c-Fos Antibody, 1:1,000; overnight at 4 °C, HY-P80616), anti-Ctsk (1:1,000, HY-P81163) and anti-MMP9 (1:1,000).

  • c-Fos Antibody Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg) using c-Fos Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • c-Fos Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using c-Fos Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • c-Fos Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • c-Fos Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • c-Fos Antibody Product Image WB
  • c-Fos Antibody Product Image WB
  • c-Fos Antibody Product Image IHC-P
  • c-Fos Antibody Product Image IHC-P