2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Alpha B Crystallin Antibody (YA1292)

Alpha B Crystallin Antibody (YA1292)

Cat. No.: HY-P81547
User Guide for Antibodies Technical Support

Alpha B Crystallin Antibody (YA1292) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Alpha B Crystallin.

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Alpha B Crystallin Antibody (YA1292) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Alpha B Crystallin.
Host

Rabbit
Clonality

Monoclonal, Recombinant
Molecular Weight
Predicted band size: 20kDa;
Observed band size: 22kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human CRYAB. The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:100-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
IP
IP: Immunoprecipitation
1:20-50
Sensitivity Endogenous Purity affinity purified by Protein A
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity)
Subcellular Localization：Cytoplasm; Nucleus; Secreted; Lysosome
Expression：
Tissue_specificity:It is expressed in the lens and other tissues (PubMed:2387586, PubMed:838078) . It is also expressed in myocardial tissue (PubMed:28493373) .
Positive sample: Rat heart tissue lysate, human skeletal muscle tissue lysate, Hela, human embryonic skeletal muscle tissue, human prostate tissue.
Subunit：Heteromer composed of three CRYAA and one CRYAB subunits (PubMed:20836128). Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes (PubMed:10751411). Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens (PubMed:22890888). Interacts with HSPBAP1 and TTN/titin (PubMed:14676215). Interacts with TMEM109; in the cellular response to DNA damage (PubMed:23542032). Interacts with DES; binds rapidly during early stages of DES filament assembly and a reduced binding seen in the later stages (PubMed:28470624). Interacts with TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059). Interacts with ATP6V1A and with MTOR, forming a ternary complex (PubMed:31786107)
Database

Entrez Gene: 1410 Human ; 25420 Rat

SwissProt: P02511 Human ; P23928 Rat

OMIM: 608810 Human

Synonyms
HspB5, CRYAB, CRYA2, HSPB5
Documentation
User Guide for Antibodies (1077 KB)

Alpha B Crystallin Antibody (YA1292) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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