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Alpha B Crystallin Antibody (YA1292)

(Synonyms: HspB5, CRYAB, CRYA2, HSPB5)
Customer Review

Based on 1 Customer Validation

Alpha B Crystallin Antibody (YA1292) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Alpha B Crystallin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, IP

  • Reactivity :

    Human, Rat, Mouse

  • Formulation:

    Supplied in10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Alpha B Crystallin Antibody (YA1292)
  • Alpha B Crystallin Antibody (YA1292) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:50-200 1:100-500 1:20-50

Product Details

Description

Alpha B Crystallin Antibody (YA1292) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Alpha B Crystallin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Rat, Mouse
  • Observed Molecular Weight
    Observed band size: 22kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 20kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CRYAB. The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Alpha B Crystallin Antibody (YA1292)
    Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2) using Alpha B Crystallin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Alpha B Crystallin may contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome

  • Subcellular Localization

    Cytoplasm; Nucleus; Secreted; Lysosome

  • Expression


    Tissue_specificity:It is expressed in the lens and other tissues (PubMed:2387586, PubMed:838078) . It is also expressed in myocardial tissue (PubMed:28493373) .
    Positive sample: Rat heart tissue lysate, human skeletal muscle tissue lysate, Hela, human embryonic skeletal muscle tissue, human prostate tissue.

  • Subunit

    Heteromer composed of three CRYAA and one CRYAB subunits (PubMed:20836128). Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes (PubMed:10751411). Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens (PubMed:22890888). Interacts with HSPBAP1 and TTN/titin (PubMed:14676215). Interacts with TMEM109; in the cellular response to DNA damage (PubMed:23542032). Interacts with DES; binds rapidly during early stages of DES filament assembly and a reduced binding seen in the later stages (PubMed:28470624). Interacts with TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059). Interacts with ATP6V1A and with MTOR, forming a ternary complex (PubMed:31786107)

  • SwissProt ID

    P02511

  • Gene ID
    1410 [NCBI]
  • Synonyms

    HspB5, CRYAB, CRYA2, HSPB5

Alpha B Crystallin Antibody (YA1292) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2) using Alpha B Crystallin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Alpha B Crystallin Antibody (YA1292) Product Image
    1/16
  • Experimental Validation Results for Alpha B Crystallin Antibody (YA1292)
  • Alpha B Crystallin Antibody (YA1292) Product Image
  • Experimental Validation Results for Alpha B Crystallin Antibody (YA1292)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2) using Alpha B Crystallin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Alpha B Crystallin Antibody (YA1292)
  • Alpha B Crystallin Antibody (YA1292) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2) using Alpha B Crystallin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Alpha B Crystallin Antibody (YA1292) Product Image WB