Ron Antibody (YA5046)
(Synonyms: MST1R; PTK8; RON; Macrophage-stimulating protein receptor; MSP receptor; CDw136; Protein-tyrosine kinase 8; p185-Ron; CD antigen CD136)Ron Antibody (YA5046) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Ron.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, IP, IF-Tissue
-
Reactivity :
Human
-
Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-200 | 1:50-200 | 1:50-200 | 1-2μg/sample |
Product Details
Ron Antibody (YA5046) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Ron.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 152 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 152 kDa
A synthesized peptide derived from human Ron (aa10-60). The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Ron receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand
-
Subcellular Localization
Membrane; Single-pass type I membrane protein
-
Expression
Tissue_specificity:It is expressed in the colon, skin, lungs, and bone marrow. -
Isoforms & Post-Translational Modification
Q04912 has 7 isomers: Q04912-1: 152241 Da (predicted); Q04912-2: 147247 Da (predicted); Q04912-3: 52519 Da (predicted); Q04912-4: 57721 Da (predicted); Q04912-5: 97315 Da (predicted); Q04912-6: 69265 Da (predicted); Q04912-7: 140697 Da (predicted).
Proteolytic processing yields the two subunits;Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site;Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation;O-mannosylation of IPT/TIG domains on Thr or Ser residues by TMEM260 is required for protein maturation (PubMed:37186866). O-mannosylated residues are composed of single mannose glycans that are not elongated or modified (PubMed:37186866) -
Subunit
Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion
-
SwissProt ID
-
Synonyms
MST1R; PTK8; RON; Macrophage-stimulating protein receptor; MSP receptor; CDw136; Protein-tyrosine kinase 8; p185-Ron; CD antigen CD136
Documentation
Powered by Bioz
