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  4. ALDOA Antibody (YA2037)

ALDOA Antibody (YA2037) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDOA.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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Description

ALDOA Antibody (YA2037) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDOA.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight
Predicted band size: 39 kDa;
Observed band size: 39 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Aldolase

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
ALL IHC-P mIHC
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human ovarian cancer tissue using ALDOA Antibody (YA2037). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82292, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background
Function:Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also function as scaffolding protein (By similarity)
Subcellular Localization:Cytoplasm, myofibril, sarcomere, I band; Cytoplasm, myofibril, sarcomere, M line
Isoforms & Post-Translational Modification:P04075 has 2 isomers: P04075-1: 39420 Da (predicted); P04075-2: 45261 Da (predicted).
Subunit:Homotetramer. Interacts with SNX9 and WAS (By similarity). Interacts with FBP2; the interaction blocks FBP2 inhibition by physiological concentrations of AMP and reduces inhibition by Ca(2+)
RRID
Database
Research Field

Signal Transduction

Synonyms
ALDOA; ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase
Documentation

ALDOA Antibody (YA2037) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ALDOA Antibody (YA2037)
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HY-P82292
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