STING Antibody

Cat. No.: HY-P80940: Data Sheet COA
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STING Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to STING.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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STING Antibody Featured Recommendations:

1 Publications Citing Use of MCE STING Antibody

•J Nanobiotechnology. 2025 May 31;23(1):403. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

STING Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to STING.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q86WV6

Gene ID

340061 [NCBI]

Immunogen

Synthetic peptide corresponding to Human STING.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH 7.3), 0.5% BSA and 50% glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HT-1080 (lane 1, 15 μg), and HT-29 (lane 2, 15 μg) using STING Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:18724357, PubMed:18818105, PubMed:19433799, PubMed:19776740, PubMed:23027953, PubMed:23747010, PubMed:23910378, PubMed:27801882, PubMed:29973723, PubMed:30842659, PubMed:35045565, PubMed:35388221, PubMed:36808561, PubMed:37832545, PubMed:25704810, PubMed:39255680). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (PubMed:26300263). Acts by binding cyclic dinucleotides: recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (PubMed:21947006, PubMed:23258412, PubMed:23707065, PubMed:23722158, PubMed:23747010, PubMed:23910378, PubMed:26229117, PubMed:30842659, PubMed:35388221, PubMed:37379839). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state (PubMed:22394562, PubMed:25636800, PubMed:29973723, PubMed:30842653, PubMed:35045565, PubMed:35388221). Exhibits 2',3' phosphodiester linkage-specific ligand recognition: can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed:23747010, PubMed:23910378, PubMed:26300263). The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (PubMed:26150511). In addition to promote the production of type I interferons, plays a direct role in autophagy (PubMed:30568238, PubMed:30842662). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (PubMed:30842662). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (PubMed:30842662). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (PubMed:37535724). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (PubMed:30568238, PubMed:30842662). Autophagy is also triggered upon infection by bacteria: following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (By similarity). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (PubMed:18724357). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (By similarity); (Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed:26405230). Such oncoproteins prevent the ability to sense cytosolic DNA (PubMed:26405230)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region; Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein; Mitochondrion outer membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Widely expressed (PubMed:18724357, PubMed:18818105) . Expressed in skin endothelial cells, type II alveolar cells, bronchial epithelial cells, and alveolar macrophages (PubMed:25029335) .

Subunit：Homodimer; forms a homodimer in absence of cyclic nucleotide (c-di-GMP or cGAMP); 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization (PubMed:19285439, PubMed:22579474, PubMed:22705373, PubMed:22728658, PubMed:22728659, PubMed:22728660, PubMed:30842659). Homotetramer; in presence of cyclic nucleotide (c-di-GMP or cGAMP), forms tetramers and higher-order oligomers through side-by-side packing (PubMed:30842653, PubMed:35388221). Interacts (when phosphorylated) with IRF3; following activation and phosphorylation on the pLxIS motif by TBK1, recruits IRF3 (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:28331227). Interacts with RIGI, MAVS and SSR2 (PubMed:18724357, PubMed:18818105). Interacts with RNF5 and TRIM56 (PubMed:19285439, PubMed:21074459). Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta (PubMed:19416887). Interacts with IFIT1 and IFIT2 (PubMed:19416887). Interacts with TRIM29; this interaction induces STING1 ubiquitination and subsequent degradation (PubMed:29038422). Associates with the MHC-II complex (By similarity). Interacts with STEEP1; interaction takes place upon cGAMP-activation and STING1 phosphorylation by MAP3K7/TAK1 and promotes STING1 translocation to COPII vesicles (PubMed:32690950, PubMed:37832545). Interacts with SEC24A, SEC24B, and SEC24C; promoting translocation to COPII vesicles (PubMed:30842662, PubMed:32690950). Interacts (when ubiquitinated) with SQSTM1; leading to relocalization to autophagosomes (By similarity). Interacts with SURF4 (PubMed:29251827). Interacts with HNRNPA2B1 (PubMed:31320558). Interacts with ZDHHC1; ZDHHC1 constitutively interacts with STING1 and in presence of DNA viruses activates it by promoting its cGAMP-induced oligomerization and the recruitment of downstream signaling components (PubMed:25299331). Interacts with ZDHHC11; in presence of DNA viruses promotes the recruitment of IRF3 to STING1 (PubMed:28331227). Interacts with TOMM70 (PubMed:20628368). Interacts with isoform IFI16-beta of IFI16 (PubMed:30104205). Interacts with TAB1; promoting recruitment of TAB1 to the endoplasmic reticulum membrane and subsequent activation of MAP3K7/TAK1 (PubMed:37832545). Interacts (via transmembrane domain) with TMEM203 (By similarity). Interacts with DDX41 (PubMed:25704810). Interacts with TMEM120A (via C-terminal domain); regulates the trafficking of STING1 from the ER to the ER-Golgi intermediate compartment to elicit antiviral effects (PubMed:35013224)

RRID

AB_3101991

Database

Entrez Gene: 340061 Human ; 72512 Mouse ;

SwissProt: Q86WV6 Human ; Q3TBT3 Mouse ;

OMIM: 615934 Human

Research Field

Immunology

Synonyms

ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; STING-beta; Transmembrane Protein 173

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

STING Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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STING Antibody

STING Antibody Featured Recommendations:

1 Publications Citing Use of MCE STING Antibody

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Background

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STING Antibody Related Classifications

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