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Cardiac Troponin T Antibody (YA4031)

(Synonyms: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6)
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Based on 1 Customer Validation

Cardiac Troponin T Antibody (YA4031) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cardiac Troponin T.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cardiac Troponin T Antibody (YA4031)
  • Cardiac Troponin T Antibody (YA4031) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Cardiac Troponin T Antibody (YA4031) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cardiac Troponin T.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 40-50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Immunogen

Purified recombinant fragment of human TNNT2 (AA: full 1-295) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cardiac Troponin T Antibody (YA4031)
    Immunohistochemical analysis of paraffin-embedded human heart muscle using Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84334, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cardiac Troponin T is a Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity

  • Expression


    Tissue_specificity:Heart. In the fetal heart, the atria are expressed more abundantly than the ventricles, while in the human heart, the ventricles are expressed more abundantly than the atria. Isotype 6 is dominant in the normal human heart. Isotypes 1, 7, and 8 are expressed in the fetal heart. Isotype 7 is also expressed in the failing human heart.

  • Isoforms & Post-Translational Modification

    P45379 has 12 isomers: P45379-1: 35924 Da (predicted); P45379-2: 35794 Da (predicted); P45379-3: 35794 Da (predicted); P45379-4: 35665 Da (predicted); P45379-5: 35795 Da (predicted); P45379-6: 34590 Da (predicted); P45379-7: 34091 Da (predicted); P45379-8: 35424 Da (predicted); P45379-9: 31137 Da (predicted); P45379-10: 35596 Da (predicted); P45379-11: 34263 Da (predicted); P45379-12: 30681 Da (predicted).
    Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity

  • SwissProt ID

    P45379

  • Gene ID
    7139 [NCBI]
  • Synonyms

    CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6

Cardiac Troponin T Antibody (YA4031) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle using Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84334, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cardiac Troponin T Antibody (YA4031) Product Image
    1/16
  • Experimental Validation Results for Cardiac Troponin T Antibody (YA4031)
  • Cardiac Troponin T Antibody (YA4031) Product Image
  • Experimental Validation Results for Cardiac Troponin T Antibody (YA4031)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle using Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84334, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cardiac Troponin T Antibody (YA4031)
  • Cardiac Troponin T Antibody (YA4031) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle using Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P84334, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cardiac Troponin T Antibody (YA4031) Product Image IHC-P