Slow Skeletal Myosin Heavy chain Antibody (YA6787)

Cat. No.: HY-P87094: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Slow Skeletal Myosin Heavy chain Antibody (YA6787) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slow Skeletal Myosin Heavy chain.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Slow Skeletal Myosin Heavy chain Antibody (YA6787) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slow Skeletal Myosin Heavy chain.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 223 kDa;

Observed band size: 223 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P12883

Gene ID

4625 [NCBI]

Immunogen

Synthetic peptide within human MYH7 aa 1,250-1,300.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded rat heart tissue using Slow Skeletal Myosin Heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Slow Skeletal Myosin Heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle

Subcellular Localization：Cytoplasm, myofibril,Cytoplasm, myofibril, sarcomere

Expression：
Tissue_Specificity: Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level)

Isoforms & Post-Translational Modification：P12883: 1935 amino acids, molecular weight 223097 Da.

Subunit：Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)

RRID

AB_3719259

Synonyms

Beta myosin heavy chain antibody; cardiac muscle beta isoform antibody; CMD1S antibody; CMH1 antibody; MPD1 antibody; MYH7 antibody; MYH7_HUMAN antibody; Myhc slow antibody; MyHC-beta antibody; MyHC-slow antibody; Beta myosin heavy chain antibody; cardiac muscle beta isoform antibody; CMD1S antibody; CMH1 antibody; MPD1 antibody; MYH7 antibody; MYH7_HUMAN antibody; Myhc slow antibody; MyHC-beta antibody; MyHC-slow antibody; MYHCB antibody; Myopathy, distal 1 antibody; Myosin heavy chain (AA 1-96) antibody; Myosin heavy chain 7 antibody; Myosin heavy chain antibody; Myosin heavy chain slow isoform antibody; Myosin heavy chain, cardiac muscle beta isoform antibody; Myosin, heavy chain 7, cardiac muscle, beta antibody; Myosin, heavy polypeptide 7, cardiac muscle, beta antibody; Myosin-7 antibody; Rhabdomyosarcoma antigen MU RMS 40.7A antibody; SPMD antibody; SPMM antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)