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RRAS Antibody (YA3212)

(Synonyms: p23)
Customer Review

Based on 1 Customer Validation

RRAS Antibody (YA3212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRAS.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RRAS Antibody (YA3212)
  • RRAS Antibody (YA3212) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

RRAS Antibody (YA3212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRAS.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database

Entrez Gene: 6237 Human

SwissProt: P10301 Human

Immunogen

A synthetic peptide of human RRAS

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105032

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RRAS Antibody (YA3212)
    Western blot analysis of extracts from HT-29(lane 2(20ug)，NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using RRAS Antibody (HY-P83467) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    RRAS is a GTP-binding protein with GTPase activity, likely involved in the regulation of MAPK signaling pathway and thereby controlling multiple cellular processes. Regulates the organization of the actin cytoskeleton. With OSPBL3, modulates integrin beta-1 (ITGB1) activity[1][2][3].

  • Subcellular Localization

    Cell membrane; Lipid-anchor; Cytoplasmic side

  • Subunit

    Interacts with PLCE1 (PubMed:16537651). Interacts with ZDHHC19 (PubMed:20074548)

  • SwissProt ID

    P10301

  • Gene ID
    6237 [NCBI]
  • Synonyms

    p23

  • Research Field

    Cell Biology

RRAS Antibody (YA3212) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HT-29(lane 2(20ug)，NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using RRAS Antibody (HY-P83467) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RRAS Antibody (YA3212) Product Image
    1/16
  • Experimental Validation Results for RRAS Antibody (YA3212)
  • RRAS Antibody (YA3212) Product Image
  • Experimental Validation Results for RRAS Antibody (YA3212)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HT-29(lane 2(20ug)，NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using RRAS Antibody (HY-P83467) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RRAS Antibody (YA3212)
  • RRAS Antibody (YA3212) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HT-29(lane 2(20ug)，NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using RRAS Antibody (HY-P83467) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RRAS Antibody (YA3212) Product Image WB