IGJ Antibody (YA1290)

Cat. No.: HY-P81545: Ficha de datos COA
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IGJ Antibody (YA1290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGJ.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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IGJ Antibody (YA1290) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

IGJ Antibody (YA1290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGJ.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 18 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P01591

Gene ID

3512 [NCBI]

Immunogen

Synthetic peptide within Human IGJ aa 50-150 (internal sequence).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000
FC FC: Flow Cytometry	1:500-1:1000

Sensitivity	Endogenous	Pureza	Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Ramos (lane 2(20μg) and Ramos (lane 3(40μg) IGJ (HY-P81545) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component

Subcellular Localization：Secreted

Subunit：Part of the secretory IgA (sIgA) complex that consists of two, four or five IgA monomers, and two additional non-Ig polypeptides, namely the JCHAIN and the secretory component (the proteolytic product of PIGR) (PubMed:32029686). Part of the secretory IgM (sIgM) complex that consists of five IgM monomers, and two additional non-Ig polypeptides, namely the JCHAIN and the secretory component (the proteolytic product of PIGR) (PubMed:32029686, PubMed:32029689). JCHAIN-containing IgM interacts (via CH4 domain) with FCRM (via Ig-like domain) (PubMed:37095205)

RRID

AB_3103657

Database

Entrez Gene: 3512 Human ; 16069 Mouse ;

SwissProt: P01591 Human ; P01592 Mouse ;

OMIM: 147790 Human

Research Field

Cell biology

Synonyms

IGCJ antibody; IGJ_HUMAN antibody; Immunoglobulin J chain antibody; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody; immunoglobulin joining chain antibody; JCH antibody; JCHAIN antibody; Joining chain of multimeric IgA and IgM antibody

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

IGJ Antibody (YA1290) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IGJ Antibody (YA1290)

IGJ Antibody (YA1290) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

IGJ Antibody (YA1290) Related Classifications

Productos vistos recientemente:

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