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CXCR5 Antibody (YA2106)

(別名: CXCR5; BLR1; MDR15; C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15; MDR-15; CD185)
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Based on 1 Customer Validation

CXCR5 Antibody (YA2106) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CXCR5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • CXCR5 Antibody (YA2106) Product Image
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価格（税別）
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数量
Amount: USD 0.00
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

製品説明

CXCR5 Antibody (YA2106) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CXCR5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CXCR5 aa330-372/372.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104470

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • 濃度

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • 輸送条件

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using CXCR5 Antibody (HY-P82361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    Flow cytometric analysis of 1X106 Raji cells labeling CXCR5 Antibody (HY-P82361, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CXCR5 is a Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expression in mature B cells and Burkitt lymphoma cells

  • Isoforms & Post-Translational Modification

    P32302 has 2 isomers: P32302-1: 41955 Da (predicted); P32302-2: 36676 Da (predicted).

  • SwissProt ID

    P32302

  • 遺伝子ID
    643 [NCBI]
  • 別名

    CXCR5; BLR1; MDR15; C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15; MDR-15; CD185

  • Research Field

    Cardiovascular

CXCR5 Antibody (YA2106) 関連分類

MOQ
Minimum order quantity
100 mg

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Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using CXCR5 Antibody (HY-P82361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Raji cells labeling CXCR5 Antibody (HY-P82361, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CXCR5 Antibody (YA2106) Product Image
    1/16
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • CXCR5 Antibody (YA2106) Product Image
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using CXCR5 Antibody (HY-P82361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Raji cells labeling CXCR5 Antibody (HY-P82361, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • Experimental Validation Results for CXCR5 Antibody (YA2106)
  • CXCR5 Antibody (YA2106) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using CXCR5 Antibody (HY-P82361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CXCR5 Antibody (YA2106) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Raji cells labeling CXCR5 Antibody (HY-P82361, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CXCR5 Antibody (YA2106) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CXCR5 Antibody (YA2106) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with CXCR5 Antibody (HY-P82361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CXCR5 Antibody (HY-P82361) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CXCR5 Antibody (YA2106) Product Image WB
  • CXCR5 Antibody (YA2106) Product Image FC
  • CXCR5 Antibody (YA2106) Product Image ICC
  • CXCR5 Antibody (YA2106) Product Image ICC