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Rat IgG1 Isotype Control Antibody (YA8865)

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Based on 1 publication(s) in Google Scholar

Rat IgG1 Isotype Control Antibody (YA8865) is a Non-conjugated and Rat origined monoclonal antibody, It can be used as an Rat IgG1 isotype control.

For research use only. We do not sell to patients.
  • Host:

    Rat

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue, FC, ICC/IF, ELISA

  • Reactivity :

    species independent

  • Formulation:

    Supplied in 0.01M PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Rat IgG1 Isotype Control Antibody (YA8865)
  • Rat IgG1 Isotype Control Antibody (YA8865) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent Assay-dependent

Product Details

Description

Rat IgG1 Isotype Control Antibody (YA8865) is a Non-conjugated and Rat origined monoclonal antibody, It can be used as an Rat IgG1 isotype control.

  • Host Rat
  • Species Reactivity
    species independent
Purification

Protein A/G purified

Conjugation

Non-conjugated

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rat IgG1 Isotype Control Antibody (YA8865)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2, Negative), Jurkat (lane 3, Negative), U87 (lane 4, Negative), and Rat brain (lane 5, Negative) using Rat IgG1 Isotype Control antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Isotype control antibody: Its main function is as a negative control, used to assess non-specific binding and background fluorescence in experiments.

Rat IgG1 Isotype Control Antibody (YA8865) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2, Negative), Jurkat (lane 3, Negative), U87 (lane 4, Negative), and Rat brain (lane 5, Negative) using Rat IgG1 Isotype Control antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Rat IgG1 Isotype Control Antibody (YA8865) Product Image
    1/16
  • Experimental Validation Results for Rat IgG1 Isotype Control Antibody (YA8865)
  • Rat IgG1 Isotype Control Antibody (YA8865) Product Image
  • Experimental Validation Results for Rat IgG1 Isotype Control Antibody (YA8865)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2, Negative), Jurkat (lane 3, Negative), U87 (lane 4, Negative), and Rat brain (lane 5, Negative) using Rat IgG1 Isotype Control antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Rat IgG1 Isotype Control Antibody (YA8865)
  • Rat IgG1 Isotype Control Antibody (YA8865) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2, Negative), Jurkat (lane 3, Negative), U87 (lane 4, Negative), and Rat brain (lane 5, Negative) using Rat IgG1 Isotype Control antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Rat IgG1 Isotype Control Antibody (YA8865) Product Image WB