Secretin Receptor Antibody
(Synonyms: BMYB; Myb-related protein B; B-Myb; Myb-like protein 2)Secretin Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Secretin Receptor.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
Secretin Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Secretin Receptor.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
Synthetic peptide corresponding to the center region of human Secretin Receptor.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Secretin Receptor is a G protein-coupled receptor activated by secretin (SCT), which is involved in different processes such as regulation of the pH of the duodenal content, food intake and water homeostasis. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and activates cAMP-dependent pathway. Upon binding to secretin, regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas (By similarity). In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation. Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation by regulating renal water reabsorption. Also plays a role in the central nervous system: required for synaptic plasticity (By similarity)[1][2][3][4].
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Subcellular Localization
Cell membrane; Basolateral cell membrane
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Isoforms & Post-Translational Modification
Secretin Receptor has an amino acid length of 440, molecular weight is 50207 Da.
Phosphorylated on Ser and Thr residues at the cytoplasmic C-terminus by G protein-coupled receptor kinases (GRKs). -
SwissProt ID
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Synonyms
BMYB; Myb-related protein B; B-Myb; Myb-like protein 2
Documentation
References
[1]. Afroze S, et al. The physiological roles of secretin and its receptor. Ann Transl Med. 2013 Oct;1(3):29. [Content Brief]
[2]. Dong M, et al. Structure and dynamics of the active Gs-coupled human secretin receptor. Nat Commun. 2020 Aug 18;11(1):4137. [Content Brief]
[3]. Fukuhara S, et al. Structure of the human secretin receptor coupled to an engineered heterotrimeric G protein. Biochem Biophys Res Commun. 2020 Dec 17;533(4):861-866. [Content Brief]
[4]. Chow BK, et al. Molecular cloning and functional characterization of a human secretin receptor. Biochem Biophys Res Commun. 1995 Jul 6;212(1):204-11. [Content Brief]
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