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alpha Actinin 4 Antibody (YA2062)

(Synonyms: Alpha-actinin-4 ,Non-muscle alpha-actinin 4 ,ACTN4)
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Based on 1 publication(s) in Google Scholar

alpha Actinin 4 Antibody (YA2062) is a rabbit-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA2062), targeting alpha Actinin 4. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • alpha Actinin 4 Antibody (YA2062) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

alpha Actinin 4 Antibody (YA2062) is a rabbit-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA2062), targeting alpha Actinin 4. It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 105 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 105 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human alpha Actinin 4 aa1-14.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104428

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using alpha Actinin 4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    Immunocytochemistry analysis of NIH3T3 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    Immunocytochemistry analysis of A549 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    alpha Actinin 4 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation[1][2][3].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cell junction; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    O43707 has 3 isomers: O43707-1: 104854 Da (predicted); O43707-2: 79913 Da (predicted); O43707-3: 59579 Da (predicted).

  • Subunit

    Homodimer; antiparallel. Binds TRIM3 at the N-terminus (PubMed:15772161). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (PubMed:17289661). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (PubMed:15772161). Interacts with MAGI1 (PubMed:12042308). Interacts with PDLIM2 (By similarity). Interacts with PPARG and RARA (PubMed:22351778). Binds to VCL; this interaction triggers VCL conformational changes (PubMed:15988023). Interacts with SEPTIN14 (PubMed:33228246). Interacts with IGSF8 (PubMed:22689882)

  • SwissProt ID

    O43707

  • Gene ID
    81 [NCBI]
  • Synonyms

    Alpha-actinin-4 ,Non-muscle alpha-actinin 4 ,ACTN4

  • Research Field

    Signal Transduction

alpha Actinin 4 Antibody (YA2062) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using alpha Actinin 4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • alpha Actinin 4 Antibody (YA2062) Product Image
    1/16
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • alpha Actinin 4 Antibody (YA2062) Product Image
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using alpha Actinin 4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • Experimental Validation Results for alpha Actinin 4 Antibody (YA2062)
  • alpha Actinin 4 Antibody (YA2062) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using alpha Actinin 4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • alpha Actinin 4 Antibody (YA2062) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH3T3 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • alpha Actinin 4 Antibody (YA2062) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling alpha Actinin 4 with alpha Actinin 4 Antibody (HY-P82317) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Actinin 4 Antibody ((HY-P82317) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • alpha Actinin 4 Antibody (YA2062) Product Image WB
  • alpha Actinin 4 Antibody (YA2062) Product Image ICC
  • alpha Actinin 4 Antibody (YA2062) Product Image ICC