LRRFIP1 Antibody (YA5242)

Cat. No.: HY-P85550: Data Sheet COA
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LRRFIP1 Antibody (YA5242) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to LRRFIP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

LRRFIP1 Antibody (YA5242) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to LRRFIP1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q32MZ4

Gene ID

9208 [NCBI]

Immunogen

Purified recombinant human LRRFIP1 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAJI(lane 2(40μg),C2C12(lane 3(40μg), MCF-7(lane 4(40μg),JURKAT(lane 5(40μg)using LRRFIP1 Antibody (YA5242). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

Background

Function：Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：Q32MZ4 has 4 isomers: Q32MZ4-1: 89253 Da (predicted); Q32MZ4-2: 86404 Da (predicted); Q32MZ4-3: 82689 Da (predicted); Q32MZ4-4: 73060 Da (predicted).

Subunit：Homodimer. May also form higher oligomers. Interacts with FLII. Interacts with MYD88. Competes with FLII for MyD88-binding, even in the absence of LPS

Synonyms

FLAP1; FLIIAP 1; GC binding factor 2; GCF2; HUFI1; Leucine rich repeat; in FLII; interacting protein 1; Leucine rich repeat flightless interacting protein 1; LRR FLII interacting protein 1; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)