Copy Link Print Information

LRRFIP1 Antibody (YA5242)

(Synonyms: FLAP1; FLIIAP 1; GC binding factor 2; GCF2; HUFI1; Leucine rich repeat; in FLII; interacting protein 1; Leucine rich repeat flightless interacting protein 1; LRR FLII interacting protein 1; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP.)
Customer Review

Based on 1 Customer Validation

LRRFIP1 Antibody (YA5242) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to LRRFIP1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LRRFIP1 Antibody (YA5242)
  • LRRFIP1 Antibody (YA5242) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

LRRFIP1 Antibody (YA5242) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to LRRFIP1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 160 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Purified recombinant human LRRFIP1 protein fragments expressed in E.coli.

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LRRFIP1 Antibody (YA5242)
    Western blot analysis of extracts from RAJI(lane 2(40μg),C2C12(lane 3(40μg), MCF-7(lane 4(40μg),JURKAT(lane 5(40μg)using LRRFIP1 Antibody (YA5242). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

Background

  • Function

    LRRFIP1 transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    Q32MZ4 has 4 isomers: Q32MZ4-1: 89253 Da (predicted); Q32MZ4-2: 86404 Da (predicted); Q32MZ4-3: 82689 Da (predicted); Q32MZ4-4: 73060 Da (predicted).

  • Subunit

    Homodimer. May also form higher oligomers. Interacts with FLII. Interacts with MYD88. Competes with FLII for MyD88-binding, even in the absence of LPS

  • SwissProt ID

    Q32MZ4

  • Gene ID
    9208 [NCBI]
  • Synonyms

    FLAP1; FLIIAP 1; GC binding factor 2; GCF2; HUFI1; Leucine rich repeat; in FLII; interacting protein 1; Leucine rich repeat flightless interacting protein 1; LRR FLII interacting protein 1; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP.

LRRFIP1 Antibody (YA5242) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAJI(lane 2(40μg),C2C12(lane 3(40μg), MCF-7(lane 4(40μg),JURKAT(lane 5(40μg)using LRRFIP1 Antibody (YA5242). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • LRRFIP1 Antibody (YA5242) Product Image
    1/16
  • Experimental Validation Results for LRRFIP1 Antibody (YA5242)
  • LRRFIP1 Antibody (YA5242) Product Image
  • Experimental Validation Results for LRRFIP1 Antibody (YA5242)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAJI(lane 2(40μg),C2C12(lane 3(40μg), MCF-7(lane 4(40μg),JURKAT(lane 5(40μg)using LRRFIP1 Antibody (YA5242). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem
  • Experimental Validation Results for LRRFIP1 Antibody (YA5242)
  • LRRFIP1 Antibody (YA5242) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAJI(lane 2(40μg),C2C12(lane 3(40μg), MCF-7(lane 4(40μg),JURKAT(lane 5(40μg)using LRRFIP1 Antibody (YA5242). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • LRRFIP1 Antibody (YA5242) Product Image WB