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FAP Antibody (YA1090)(PBS only)

(Synonyms: FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease)

FAP Antibody (YA1090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • FAP Antibody (YA1090)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

FAP Antibody (YA1090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FAP.

  • Host Rabbit
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 88 kDa; Observed band size: 95 kDa
Immunogen

A synthesized peptide derived from human FAP1 aa1-250.

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
    Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Fibroblast activation protein (FAP) is a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP is highly upregulated in a wide variety of cancers, and is often used as a marker for pro-tumorigenic stroma.

  • Subcellular Localization

    Cell surface; Cell membrane; Single-pass type II membrane protein; Cell projection, lamellipodium membrane; Single-pass type II membrane protein; Cell projection, invadopodium membrane; Single-pass type II membrane protein; Cell projection, ruffle membrane; Single-pass type II membrane protein; Membrane; Single-pass type II membrane protein; Secreted; Cytoplasm

  • Expression


    Tissue_specificity:Expressed in adipose tissue. Expressed in dermal fibroblasts of fetal skin. Expressed in granulation tissue of healing wounds and reactive stromal fibroblasts of epithelial carcinoma. Expressed in activated fibroblast-like synovial cells in inflamed synovial tissue. Expressed in activated hepatic stellate cells (HSCs) and myofibroblasts in cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (protein level) . Expressed in glioblastoma and glioma cells. Isomer 1 and isomer 2 are expressed in melanoma, carcinoma, and fibroblast cell lines.

    Induction:In fibroblasts at times and sites of tissue remodeling during development, tissue repair and carcinogenesis. Up-regulated upon tumor stem cell differentiation. Up-regulated by transforming growth factor-beta, 12-O-tetradecanoyl phorbol-13-acetate and retinoids

  • Isoforms & Post-Translational Modification

    Q12884 has 2 isomers: Q12884-1: 87713 Da (predicted); Q12884-2: 26954 Da (predicted).
    N-glycosylated;The N-terminus may be blocked

  • Subunit

    Homodimer; homodimerization is required for activity of both plasma membrane and soluble forms. The monomer is inactive. Heterodimer with DPP4. Interacts with PLAUR; the interaction occurs at the cell surface of invadopodia membranes. Interacts with ITGB1. Interacts with ITGA3. Associates with integrin alpha-3/beta-1; the association occurs in a collagen-dependent manner at the cell surface of invadopodia membranes

  • SwissProt ID

    Q12884

  • Gene ID
    2191 [NCBI]
  • Synonyms

    FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease

  • Research Field

    Cell Biology

FAP Antibody (YA1090)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FAP Antibody (YA1090)(PBS only) Product Image
    1/16
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • FAP Antibody (YA1090)(PBS only) Product Image
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • Experimental Validation Results for FAP Antibody (YA1090)(PBS only)
  • FAP Antibody (YA1090)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FAP Antibody (YA1090)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FAP Antibody (YA1090)(PBS only) Product Image WB
  • FAP Antibody (YA1090)(PBS only) Product Image WB