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FAP Antibody (YA1090)

(Synonyms: FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease)
Customer Review

Based on 1 Customer Validation

FAP Antibody (YA1090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • FAP Antibody (YA1090) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

FAP Antibody (YA1090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 88 kDa; Observed band size: 95 kDa
Species Reactivity Database

Entrez Gene: 2191 Human

SwissProt: Q12884 Human

Immunogen

A synthesized peptide derived from human FAP1 aa1-250.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103460

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FAP Antibody (YA1090)
    Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human prostate tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    Immunohistochemical analysis of paraffin-embedded human soft tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Fibroblast activation protein (FAP) is a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP is highly upregulated in a wide variety of cancers, and is often used as a marker for pro-tumorigenic stroma.

  • Subcellular Localization

    Cell surface; Cell membrane; Single-pass type II membrane protein; Cell projection, lamellipodium membrane; Single-pass type II membrane protein; Cell projection, invadopodium membrane; Single-pass type II membrane protein; Cell projection, ruffle membrane; Single-pass type II membrane protein; Membrane; Single-pass type II membrane protein; Secreted; Cytoplasm

  • Expression


    Tissue_specificity:Expressed in adipose tissue. Expressed in dermal fibroblasts of fetal skin. Expressed in granulation tissue of healing wounds and reactive stromal fibroblasts of epithelial carcinoma. Expressed in activated fibroblast-like synovial cells in inflamed synovial tissue. Expressed in activated hepatic stellate cells (HSCs) and myofibroblasts in cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (protein level) . Expressed in glioblastoma and glioma cells. Isomer 1 and isomer 2 are expressed in melanoma, carcinoma, and fibroblast cell lines.

    Induction:In fibroblasts at times and sites of tissue remodeling during development, tissue repair and carcinogenesis. Up-regulated upon tumor stem cell differentiation. Up-regulated by transforming growth factor-beta, 12-O-tetradecanoyl phorbol-13-acetate and retinoids

  • Isoforms & Post-Translational Modification

    Q12884 has 2 isomers: Q12884-1: 87713 Da (predicted); Q12884-2: 26954 Da (predicted).
    N-glycosylated;The N-terminus may be blocked

  • Subunit

    Homodimer; homodimerization is required for activity of both plasma membrane and soluble forms. The monomer is inactive. Heterodimer with DPP4. Interacts with PLAUR; the interaction occurs at the cell surface of invadopodia membranes. Interacts with ITGB1. Interacts with ITGA3. Associates with integrin alpha-3/beta-1; the association occurs in a collagen-dependent manner at the cell surface of invadopodia membranes

  • SwissProt ID

    Q12884

  • Gene ID
    2191 [NCBI]
  • Synonyms

    FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease

  • Research Field

    Cell Biology

FAP Antibody (YA1090) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    1/16
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • FAP Antibody (YA1090) Product Image
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • Experimental Validation Results for FAP Antibody (YA1090)
  • FAP Antibody (YA1090) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using FAP Antibody (HY-P81345) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), and U251MG (lane 3, 15 μg) using FAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human soft tissue using FAP Antibody (HY-P81345, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FAP Antibody (YA1090) Product Image WB
  • FAP Antibody (YA1090) Product Image WB
  • FAP Antibody (YA1090) Product Image IHC-P
  • FAP Antibody (YA1090) Product Image IHC-P
  • FAP Antibody (YA1090) Product Image IHC-P
  • FAP Antibody (YA1090) Product Image IHC-P
  • FAP Antibody (YA1090) Product Image IHC-P
  • FAP Antibody (YA1090) Product Image IHC-P