RANK Antibody (YA4604)(PBS only)

Cat. No.: HY-P84907A: Data Sheet User Guide for Antibodies
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RANK Antibody (YA4604) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RANK.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RANK Antibody (YA4604) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RANK.

Host

Mouse

Molecular Weight

Predicted band size: 66 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y6Q6

Gene ID

8792 [NCBI]

Immunogen

Purified recombinant fragment of human TNFRSF11A (AA: extra 30-212) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed:9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (By similarity). Involved in the regulation of interactions between T-cells and dendritic cells (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:This gene is widely expressed in various tissues throughout the body, with high expression levels in skeletal muscle, thymus, liver, colon, small intestine, and adrenal glands.

Isoforms & Post-Translational Modification：Q9Y6Q6 has 6 isomers: Q9Y6Q6-1: 66034 Da (predicted); Q9Y6Q6-2: 32318 Da (predicted); Q9Y6Q6-3: 36399 Da (predicted); Q9Y6Q6-4: 28965 Da (predicted); Q9Y6Q6-5: 56402 Da (predicted); Q9Y6Q6-6: 64552 Da (predicted).

Subunit：Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer (By similarity). Part of a complex composed of EEIG1, TNFRSF11A/RANK, PLCG2, GAB2, TEC and BTK; complex formation increases in the presence of TNFSF11/RANKL (PubMed:23478294). Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 (PubMed:9774460). Interacts (via cytoplasmic domain) with GAB2 (PubMed:15750601). Interacts (via cytoplasmic domain); with EEIG1 (via N-terminus); when in the presence of TNFSF11/RANKL (By similarity)

Synonyms

FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1; TRANCE-R

Documentation

User Guide for Antibodies (1077 KB)