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Annexin A1/ANXA1 Antibody (YA832)

(Synonyms: ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35)
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Based on 1 Customer Validation

Annexin A1/ANXA1 Antibody (YA832) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Annexin A1/ANXA1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Annexin A1/ANXA1 Antibody (YA832) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Annexin A1/ANXA1 Antibody (YA832) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Annexin A1/ANXA1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Annexin A1 beta.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102600

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HCT 116 (lane 3), NIH/3T3 (lane 4), K562 (lane 5) and Rat kidney (lane 6) using Annexin A1/ANXA1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
    Western blot analysis of extracts from A549(lane 2(20μg) 、Hela (lane 3（20μg) and C6(lane 4(20μg)), using Annexin A1 (HY-P80544) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80544, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Annexin A1/ANXA1 plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton; Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cell projection, cilium; Cell membrane; Membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Basolateral cell membrane; Apical cell membrane; Lateral cell membrane; Secreted; Secreted, extracellular space; Cell membrane; Peripheral membrane protein; Extracellular side; Secreted, extracellular exosome; Cytoplasmic vesicle, secretory vesicle lumen; Cell projection, phagocytic cup; Early endosome; Cytoplasmic vesicle membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:Detected in resting neutrophils (PubMed: 10772777) . Detected in peripheral blood T cells (PubMed: 17008549) . Detected in extracellular vesicles of inflammatory bowel disease serum, but not detected in serum of healthy donors (PubMed: 25664854) . Detected in placenta (protein level) (PubMed: 2532504) . Detected in liver.

  • Subunit

    Homodimer; non-covalently linked (By similarity). Homodimer; linked by transglutamylation (PubMed:2532504).

  • SwissProt ID

    P04083

  • Gene ID
    301 [NCBI]
  • Synonyms

    ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

  • Research Field

    Signal Transduction

[1]. Arcone R, et al. Structural characterization of a biologically active human lipocortin 1 expressed in Escherichia coli. Eur J Biochem. 1993 Jan 15;211(1-2):347-55. [Content Brief]

[2]. D'Acquisto F, et al. Annexin-1 modulates T-cell activation and differentiation. Blood. 2007 Feb 1;109(3):1095-102. [Content Brief]

[3]. McArthur S, et al. Annexin A1 regulates hormone exocytosis through a mechanism involving actin reorganization. FASEB J. 2009 Nov;23(11):4000-10. [Content Brief]

[4]. Pepinsky RB, et al. A dimeric form of lipocortin-1 in human placenta. Biochem J. 1989 Oct 1;263(1):97-103. [Content Brief]

[5]. Mailliard WS, et al. Calcium-dependent binding of S100C to the N-terminal domain of annexin I. J Biol Chem. 1996 Jan 12;271(2):719-25. [Content Brief]

[6]. Ernst S, et al. An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor family. J Immunol. 2004 Jun 15;172(12):7669-76. [Content Brief]

[7]. Woloszynek JC, et al. Cathepsin G-regulated release of formyl peptide receptor agonists modulate neutrophil effector functions. J Biol Chem. 2012 Oct 5;287(41):34101-9. [Content Brief]

[8]. Leoni G, et al. Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. J Clin Invest. 2015 Mar 2;125(3):1215-27. [Content Brief]

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Annexin A1/ANXA1 Antibody (YA832) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HCT 116 (lane 3), NIH/3T3 (lane 4), K562 (lane 5) and Rat kidney (lane 6) using Annexin A1/ANXA1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg) 、Hela (lane 3（20μg) and C6(lane 4(20μg)), using Annexin A1 (HY-P80544) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80544, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Annexin A1/ANXA1 Antibody (YA832) Product Image
    1/16
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Annexin A1/ANXA1 Antibody (YA832) Product Image
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HCT 116 (lane 3), NIH/3T3 (lane 4), K562 (lane 5) and Rat kidney (lane 6) using Annexin A1/ANXA1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg) 、Hela (lane 3（20μg) and C6(lane 4(20μg)), using Annexin A1 (HY-P80544) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80544, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Experimental Validation Results for Annexin A1/ANXA1 Antibody (YA832)
  • Annexin A1/ANXA1 Antibody (YA832) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HCT 116 (lane 3), NIH/3T3 (lane 4), K562 (lane 5) and Rat kidney (lane 6) using Annexin A1/ANXA1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Annexin A1/ANXA1 Antibody (YA832) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20μg) 、Hela (lane 3（20μg) and C6(lane 4(20μg)), using Annexin A1 (HY-P80544) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80544, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Annexin A1/ANXA1 Antibody (YA832) Product Image WB
  • Annexin A1/ANXA1 Antibody (YA832) Product Image WB