ARCN1 Antibody (YA7914)
(Synonyms: COPD)
Based on 1 Customer Validation
ARCN1 Antibody (YA7914) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARCN1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Dog, Rat, Mouse
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-500 | 1:100-250 | 1:100 |
Product Details
ARCN1 Antibody (YA7914) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARCN1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Dog, Rat, Mouse
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Calculated Molecular Weight Predicted band size: 57 kDa
Full length human recombinant protein of human ARCN1 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ARCN1 Antibody (HY-P88230). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
Background
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Function
ARCN1 component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors
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Subcellular Localization
Cytoplasm,Golgi apparatus membrane,Cytoplasmic vesicle, COPI-coated vesicle membrane
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Expression
Tissue_Specificity: Ubiquitously expressed -
Isoforms & Post-Translational Modification
P48444 has two isomers: P48444-1: 57210 Da (predicted); P48444-2: 47205 Da (predicted).
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Subunit
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits
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SwissProt ID
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Synonyms
COPD
Documentation
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