ARCN1 Antibody (YA7914)

Cat. No.: HY-P88230: Ficha de datos COA
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ARCN1 Antibody (YA7914) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARCN1.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

ARCN1 Antibody (YA7914) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARCN1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 57 kDa

Species Reactivity

Human, Dog, Rat, Mouse

SwissProt ID

P48444

Immunogen

Full length human recombinant protein of human ARCN1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ARCN1 Antibody (HY-P88230). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity)

Subcellular Localization：Cytoplasm,Golgi apparatus membrane,Cytoplasmic vesicle, COPI-coated vesicle membrane

Expression：
Tissue_Specificity: Ubiquitously expressed

Isoforms & Post-Translational Modification：P48444 has two isomers: P48444-1: 57210 Da (predicted); P48444-2: 47205 Da (predicted).

Subunit：Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits

Synonyms

COPD

Documentación

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Ficha de datos (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)