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ABCF1 Antibody (YA2712)

(Synonyms: ABC27; ABC50; ABCF1; ATP-binding cassette 50; ATP-binding cassette 50 (TNF-alpha stimulated); TNF-alpha-stimulated ABC protein)
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Based on 1 Customer Validation

ABCF1 Antibody (YA2712) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ABCF1 Antibody (YA2712)
  • ABCF1 Antibody (YA2712) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

ABCF1 Antibody (YA2712) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 96 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ABCF1 aa800-845.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105086

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ABCF1 Antibody (YA2712)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), K562 (lane 3, 15 μg), 293T (lane 4, 15 μg), Caco-2 (lane 5, 15 μg), and A431 (lane 6, 15 μg) using ABCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ABCF1 is an Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis

  • Subcellular Localization

    Cytoplasm; Nucleus, nucleoplasm; Nucleus envelope

  • Expression


    Tissue_specificity:Ubiquitous

    Induction:By TNF in cultured synoviocytes

  • Isoforms & Post-Translational Modification

    Q8NE71 has 2 isomers: Q8NE71-1: 95926 Da (predicted); Q8NE71-2: 91680 Da (predicted).
    Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes

  • Subunit

    Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes

  • SwissProt ID

    Q8NE71

  • Gene ID
    23 [NCBI]
  • Synonyms

    ABC27; ABC50; ABCF1; ATP-binding cassette 50; ATP-binding cassette 50 (TNF-alpha stimulated); TNF-alpha-stimulated ABC protein

  • Research Field

    Epigenetics and Nuclear Signaling

ABCF1 Antibody (YA2712) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), K562 (lane 3, 15 μg), 293T (lane 4, 15 μg), Caco-2 (lane 5, 15 μg), and A431 (lane 6, 15 μg) using ABCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ABCF1 Antibody (YA2712) Product Image
    1/16
  • Experimental Validation Results for ABCF1 Antibody (YA2712)
  • ABCF1 Antibody (YA2712) Product Image
  • Experimental Validation Results for ABCF1 Antibody (YA2712)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), K562 (lane 3, 15 μg), 293T (lane 4, 15 μg), Caco-2 (lane 5, 15 μg), and A431 (lane 6, 15 μg) using ABCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ABCF1 Antibody (YA2712)
  • ABCF1 Antibody (YA2712) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), K562 (lane 3, 15 μg), 293T (lane 4, 15 μg), Caco-2 (lane 5, 15 μg), and A431 (lane 6, 15 μg) using ABCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ABCF1 Antibody (YA2712) Product Image WB