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alpha smooth muscle Actin Antibody (YA627)

(Synonyms: alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin; )
6 Cited Publications
Customer Review

Based on 6 publication(s) in Google Scholar

alpha smooth muscle Actin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to alpha smooth muscle Actin. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • alpha smooth muscle Actin Antibody (YA627) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) alpha smooth muscle Actin Antibody (YA627)

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6 Publications Citing Use of MCE alpha smooth muscle Actin Antibody (YA627) (6)
IFIHCWB
  • IF
  • WB
  • WB
  • WB
  • IHC
  • IF

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

alpha smooth muscle Actin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to alpha smooth muscle Actin. It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human alpha smooth muscle Actin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102557

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    Western blot analysis of extracts from Hela (lane 2(20μg) , NIH3T3 (lane 3(20μg), A549 (lane 4(20μg) using alpha smooth muscle Actin(HY-P80484) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    Flow cytometric analysis of 1X10^6 Hela cells labeling alpha smooth muscle Actin Antibody (HY-P80484, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    alpha smooth muscle Actin is an Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Induction:Up-regulated in response to enterovirus 71 (EV71) infection

  • Subunit

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others

  • SwissProt ID

    P62736

  • Gene ID
    59 [NCBI]
  • Synonyms

    alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin;

  • Research Field

    Signal Transduction

alpha smooth muscle Actin Antibody (YA627) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , NIH3T3 (lane 3(20μg), A549 (lane 4(20μg) using alpha smooth muscle Actin(HY-P80484) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling alpha smooth muscle Actin Antibody (HY-P80484, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Customer Validation
     Immunofluorescence staining of αSMA and collagen I in fibroblasts.

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Customer Validation
     Western blot analysis of fibrosisrelated proteins in fibroblasts treated with CQ.

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Customer Validation
     Western blot analysis showing fibrosis-related protein and arginase expression after arginine inhibition treatment.

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Eur J Cell Biol. 2026 Feb 27;105(2):151534. [Abstract]

    Customer Validation
     Western blot was used to detect the expression of α-SMA, SMAD3, p-SMAD3, and OPN in normal tissue (NT) and pulmonary fibrosis (PF) cells. GAPDH was used as loading control. Quantification of the protein level shown in right.

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Cells. 2025 Sep 16;14(18):1452. [Abstract]

    Customer Validation
     Representative immunohistochemical staining and quantification of the myofibroblast marker α-smooth muscle actin (α-SMA) in myocardial tissue sections. Scale bars for whole heart sections are 0.25 mm, and for magnified views are 20 µm.

  • 1/16

    alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Res Sq. 2024 Oct 22.

    Customer Validation
     Representative immuno-histological and immuno-fluorescence staining images for CD31+ and αSMA+ densities among the 3 groups. hUC-MSCs improved fetal blood perfusion in preeclampsia rats by increasing placental angiogenesis. Ctrl, L-NAME, and L-NAME+MSC groups.

  • alpha smooth muscle Actin Antibody (YA627) Product Image
    1/16
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • alpha smooth muscle Actin Antibody (YA627) Product Image
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , NIH3T3 (lane 3(20μg), A549 (lane 4(20μg) using alpha smooth muscle Actin(HY-P80484) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling alpha smooth muscle Actin Antibody (HY-P80484, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody (YA627)

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Immunofluorescence staining of αSMA and collagen I in fibroblasts.

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Western blot analysis of fibrosisrelated proteins in fibroblasts treated with CQ.

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar 31:e15505. [Abstract]

    Western blot analysis showing fibrosis-related protein and arginase expression after arginine inhibition treatment.

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Eur J Cell Biol. 2026 Feb 27;105(2):151534. [Abstract]

    Western blot was used to detect the expression of α-SMA, SMAD3, p-SMAD3, and OPN in normal tissue (NT) and pulmonary fibrosis (PF) cells. GAPDH was used as loading control. Quantification of the protein level shown in right.

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Cells. 2025 Sep 16;14(18):1452. [Abstract]

    Representative immunohistochemical staining and quantification of the myofibroblast marker α-smooth muscle actin (α-SMA) in myocardial tissue sections. Scale bars for whole heart sections are 0.25 mm, and for magnified views are 20 µm.

  • alpha smooth muscle Actin Antibody (YA627) purchased from MedChemExpress. Usage Cited in: Res Sq. 2024 Oct 22.

    Representative immuno-histological and immuno-fluorescence staining images for CD31+ and αSMA+ densities among the 3 groups. hUC-MSCs improved fetal blood perfusion in preeclampsia rats by increasing placental angiogenesis. Ctrl, L-NAME, and L-NAME+MSC groups.

  • alpha smooth muscle Actin Antibody (YA627) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , NIH3T3 (lane 3(20μg), A549 (lane 4(20μg) using alpha smooth muscle Actin(HY-P80484) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • alpha smooth muscle Actin Antibody (YA627) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha smooth muscle Actin Antibody (YA627) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80484, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha smooth muscle Actin Antibody (YA627) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling alpha smooth muscle Actin Antibody (HY-P80484, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • alpha smooth muscle Actin Antibody (YA627) Product Image WB
  • alpha smooth muscle Actin Antibody (YA627) Product Image IHC-P
  • alpha smooth muscle Actin Antibody (YA627) Product Image IHC-P
  • alpha smooth muscle Actin Antibody (YA627) Product Image FC