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E-Cadherin Antibody (YA778)

(Synonyms: CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin)
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Based on 1 publication(s) in Google Scholar

E-Cadherin Antibody (YA778) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to E-Cadherin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • E-Cadherin Antibody (YA778) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:200

Product Details

Descripciòn

E-Cadherin Antibody (YA778) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to E-Cadherin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Mouse E-Cadherin.AA range:350-550.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102079

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using E-Cadherin (HY-P80112) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    Western blot analysis of extracts from HCT116(lane 2(20ug) , and HCT116(lane 3(40ug) using E-Cadherin Antibody (HY-P80112) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    E-Cadherin is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria[1][2].

  • Subcellular Localization

    Cell junction, adherens junction; Cell membrane; Single-pass type I membrane protein; Endosome; Golgi apparatus, trans-Golgi network; Cytoplasm; Cell junction, desmosome

  • Expression


    Tissue_specificity:Expression in granulomatous macrophages (protein level) (PubMed: 27760340) . Expression in skin (protein level) (PubMed: 22294297) . Expression in liver (PubMed: 3263290) .

    Induction:Induced in the hours following cyclic mechanical strain in keratinocytes (PubMed:31835537) . Expression is repressed by MACROD1 (PubMed:17893710)

  • Subunit

    Homodimer; disulfide-linked (PubMed:11856755). Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1.

  • SwissProt ID

    P12830

  • Gene ID
    999 [NCBI]
  • Synonyms

    CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

  • Research Field

    Signal Transduction

References

[1]. Meigs TE, et al. Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin. J Biol Chem. 2002 Jul 5;277(27):24594-600. [Content Brief]

[2]. Shafraz O, et al. E-cadherin binds to desmoglein to facilitate desmosome assembly. Elife. 2018 Jul 12;7:. [Content Brief]

E-Cadherin Antibody (YA778) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using E-Cadherin (HY-P80112) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , and HCT116(lane 3(40ug) using E-Cadherin Antibody (HY-P80112) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • E-Cadherin Antibody (YA778) Product Image
    1/16
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • E-Cadherin Antibody (YA778) Product Image
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using E-Cadherin (HY-P80112) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , and HCT116(lane 3(40ug) using E-Cadherin Antibody (HY-P80112) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • Experimental Validation Results for E-Cadherin Antibody (YA778)
  • E-Cadherin Antibody (YA778) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using E-Cadherin (HY-P80112) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • E-Cadherin Antibody (YA778) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116(lane 2(20ug) , and HCT116(lane 3(40ug) using E-Cadherin Antibody (HY-P80112) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • E-Cadherin Antibody (YA778) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • E-Cadherin Antibody (YA778) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • E-Cadherin Antibody (YA778) Product Image WB
  • E-Cadherin Antibody (YA778) Product Image WB
  • E-Cadherin Antibody (YA778) Product Image IHC-P
  • E-Cadherin Antibody (YA778) Product Image IHC-P