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EpCAM Antibody (YA772)

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Based on 1 Customer Validation

EpCAM Antibody (YA772) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to EpCAM.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgM

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EpCAM Antibody (YA772)
  • EpCAM Antibody (YA772) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200

Product Details

Beschreibung

EpCAM Antibody (YA772) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to EpCAM.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 35/38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human EP-CAM.AA range:24-265.

Sensitivity

Endogenous

Purification

Protein L affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgM

RRID

AB_3102311

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Versand

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EpCAM Antibody (YA772)
    Western blot analysis of extracts from Caco 2 (lane 1) and M-stomach (lane 2) and M-intestine (lane 3) using EpCAM antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    EpCAM (CD326) is a transmembrane glycoprotein expressed on the surface of epithelial cells and serves as a classic marker for epithelial cells and epithelial-derived tumors. It plays a role in cell adhesion, the regulation of proliferation, and the maintenance of tumor stemness, holding significant value in tumor diagnosis, circulating tumor cell detection, cancer stem cell research, and the development of targeted therapies.

  • Subcellular Localization

    Lateral cell membrane; Single-pass type I membrane protein; Cell junction, tight junction

  • Expression


    Tissue_specificity:This gene is highly and selectively expressed in undifferentiated embryonic stem cells (ESCs) , but not in differentiated ESCs. Once ESCs differentiate, its protein level rapidly declines. This gene is expressed on almost all epithelial cell membranes, but not on mesoderm or neural cell membranes. It has also been found on the surface of adenocarcinomas.

  • Subunit

    Monomer. Interacts with phosphorylated CLDN7

  • SwissProt ID

    P16422

  • Gene ID
    4072 [NCBI]
  • Research Field

    Tags & Cell Markers

EpCAM Antibody (YA772) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Caco 2 (lane 1) and M-stomach (lane 2) and M-intestine (lane 3) using EpCAM antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EpCAM Antibody (YA772) Product Image
    1/16
  • Experimental Validation Results for EpCAM Antibody (YA772)
  • EpCAM Antibody (YA772) Product Image
  • Experimental Validation Results for EpCAM Antibody (YA772)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Caco 2 (lane 1) and M-stomach (lane 2) and M-intestine (lane 3) using EpCAM antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for EpCAM Antibody (YA772)
  • EpCAM Antibody (YA772) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Caco 2 (lane 1) and M-stomach (lane 2) and M-intestine (lane 3) using EpCAM antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EpCAM Antibody (YA772) Product Image WB