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Vimentin Antibody (YA011)

9 Cited Publications
Customer Review

Based on 9 publication(s) in Google Scholar

Vimentin Antibody (YA011) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P FC
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Vimentin Antibody (YA011) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Vimentin Antibody (YA011)

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9 Publications Citing Use of MCE Vimentin Antibody (YA011) (5)
WB
  • WB
  • WB
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:20000-1:50000 1:5000-1:10000 1:1000-1:2000 1:1000-1:2000 1:100-1:500 1:1000 Use at an assay dependent concentration.

Product Details

Description

Vimentin Antibody (YA011) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Monkey, Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within C-terminal human Vimentin.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102461

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vimentin Antibody (YA011)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Vimentin(HY-P80371) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    Immunocytochemistry analysis of A549 cells labeling Vimentin with Vimentin Antibody (HY-P80371)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vimentin Antibody (YA011)
    Immunocytochemistry analysis of Hela cells labeling Vimentin with Vimentin Antibody (HY-P80371) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vimentin Antibody (YA011)
    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    Flow cytometric analysis of 1X10^6 Hela cells labeling Vimentin Antibody (HY-P80371, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity); Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Nucleus matrix; Cell membrane

  • Expression


    Tissue_specificity:It is highly expressed in fibroblasts, expressed in small amounts in T cells and B cells, and expressed very low or not at all in Burkitt lymphoma cell lines. It is expressed in many hormone-independent breast cancer cell lines.

    Induction:Up-regulated by muramyl-dipeptide and lipopolysaccharide

  • Subunit

    Homomer assembled from elementary dimers (PubMed:20176112). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with BCAS3 (PubMed:17505058). Interacts with LGSN (By similarity). Interacts with SYNM (By similarity). Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with PLEC isoform 1C (PubMed:24940650). Interacts with STK33 (PubMed:18811945). Interacts with LARP6 (PubMed:21746880). Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton (PubMed:16361107, PubMed:18827015). Interacts with TOR1AIP1 (PubMed:16361107). Interacts with DIAPH1 (PubMed:23325789). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132). Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM (PubMed:29496907). Interacts with NOD2 (PubMed:27812135). Interacts (via head region) with CORO1C (By similarity). Interacts with HDGF (isoform 2) (PubMed:26845719). Interacts with PRKCE (via phorbol-ester/DAG-type 2 domain) (PubMed:18408015). Interacts with BFSP2 (By similarity). Interacts with PPL (By similarity). Interacts (via rod domain) with PKP1 (PubMed:10852826). Interacts with PKP2 (PubMed:10852826). Interacts with SCRIB (via PDZ domains); the interaction protects SCRIB from proteasomal degradation and facilitates SCRIB localization to intermediate filaments, the interaction is reduced by cell contact inhibition (PubMed:19386766)

  • SwissProt ID

    P08670

  • Gene ID
    7431 [NCBI]
  • Research Field

    Neuroscience

Vimentin Antibody (YA011) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Vimentin(HY-P80371) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Vimentin with Vimentin Antibody (HY-P80371)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vimentin with Vimentin Antibody (HY-P80371) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Vimentin Antibody (HY-P80371, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Customer Validation
     Vimentin Antibody (YA011) (1:1000). Western blots were performed to detect the expression of vimentin in OSCC cells treated with different concentrations of glucose for 48 hours.

  • 1/16

    Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Customer Validation
     Vimentin Antibody (YA011) (1:1000). Western blot analysis of vimentin in SCC25 and CAL27 cells.

  • 1/16

    Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Customer Validation
     Vimentin Antibody (YA011) (1:1000). Western blot analysis of vimentin in OSCC cells.

  • 1/16

    Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Biochem Genet. 2024 Dec 16. [Abstract]

    Customer Validation
     Vimentin Antibody (YA011) (1:2000). Western blotting assay was used to analyze Vimentin protein expression in Huh7 and HCCLM3 cells.

  • 1/16

    Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Biochem Genet. 2024 Dec 16. [Abstract]

    Customer Validation
     Vimentin Antibody (YA011) (1:2000). Western blotting assay was used to analyze Vimentin protein expression in Huh7 and HCCLM3 cells.

  • Vimentin Antibody (YA011) Product Image
    1/16
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Vimentin Antibody (YA011) Product Image
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Vimentin(HY-P80371) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Vimentin with Vimentin Antibody (HY-P80371)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vimentin with Vimentin Antibody (HY-P80371) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vimentin Antibody (YA011)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Vimentin Antibody (HY-P80371, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)
  • Experimental Validation Results for Vimentin Antibody (YA011)

  • Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Vimentin Antibody (YA011) (1:1000). Western blots were performed to detect the expression of vimentin in OSCC cells treated with different concentrations of glucose for 48 hours.

  • Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Vimentin Antibody (YA011) (1:1000). Western blot analysis of vimentin in SCC25 and CAL27 cells.

  • Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Vimentin Antibody (YA011) (1:1000). Western blot analysis of vimentin in OSCC cells.

  • Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Biochem Genet. 2024 Dec 16. [Abstract]

    Vimentin Antibody (YA011) (1:2000). Western blotting assay was used to analyze Vimentin protein expression in Huh7 and HCCLM3 cells.

  • Vimentin Antibody (YA011) purchased from MedChemExpress. Usage Cited in: Biochem Genet. 2024 Dec 16. [Abstract]

    Vimentin Antibody (YA011) (1:2000). Western blotting assay was used to analyze Vimentin protein expression in Huh7 and HCCLM3 cells.

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Vimentin(HY-P80371) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Vimentin with Vimentin Antibody (HY-P80371)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Vimentin with Vimentin Antibody (HY-P80371) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vimentin Antibody (HY-P80371)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue using Vimentin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80371, 1/5000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Vimentin Antibody (YA011) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling Vimentin Antibody (HY-P80371, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Vimentin Antibody (YA011) Product Image WB
  • Vimentin Antibody (YA011) Product Image ICC
  • Vimentin Antibody (YA011) Product Image ICC
  • Vimentin Antibody (YA011) Product Image IHC-P
  • Vimentin Antibody (YA011) Product Image IHC-P
  • Vimentin Antibody (YA011) Product Image FC