Copy Link Print Information

IL-1 beta Antibody

(Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA)
11 Cited Publications
Customer Review

Based on 11 publication(s) in Google Scholar

IL-1 beta Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • IL-1 beta Antibody Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) IL-1 beta Antibody

More
11 Publications Citing Use of MCE IL-1 beta Antibody (5)
WBIFHistological Imaging/Staining
  • IF
  • Histological Imaging/Staining
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

IL-1 beta Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 beta.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17,31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human IL1B AA range 181-230.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102173

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-1 beta Antibody
    Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-1 beta Antibody
    Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-1 beta Antibody
    Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-1 beta Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-1 beta Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Interleukin-1β (IL-1β) is one of the pro-inflammatory cytokines and is produced and secreted by a variety of cell types although the vast majority of studies have focussed on its production within cells of the innate immune system, such as monocytes and macrophages. IL-1β is produced as inactive pro-IL-1β (encoded by pro-Il-1b) in response to inflammatory stimuli, including both microbial products and endogenous danger-associated molecules. IL-1β could regulate the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. IL-1β also plays a significant regulator of reproduction in females[1][2].

  • Subcellular Localization

    Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

  • Expression


    Tissue_specificity:Expression (protein level) in activated monocytes/macrophages.

    Induction:By LPS (PubMed:15192144) . Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components in macrophages; EsxA is the most potent activator tested (at protein level) (PubMed:20148899) . In pancreatic islets, release is increased by high glucose treatment. In pancreatic islets and macrophages, release is also increased by endocannabinoid anandamide/AEA (PubMed:23955712)

  • Isoforms & Post-Translational Modification


    1) IL1beta (P01584): Number of amino acids: 269; Molecular weight (Da): 30,748
    2) Mouse IL1beta (P10749): Number of amino acids: 269; Molecular weight (Da): 30,931
    3) Rat IL1beta (a63264): Number of amino acids: 268; Molecular weight (Da): 30,644

  • Subunit

    Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all (PubMed:17431422). Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling (PubMed:29030430). Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (PubMed:32272059). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059)

  • SwissProt ID

    P01584

  • Gene ID
    3553 [NCBI]
  • Synonyms

    IL-1; IL1F2; IL1beta; IL1-BETA

  • Research Field

    Immunology

[1]. Jan Petrasek, et al. IL-1 receptor antagonist ameliorates inflammasome-dependent alcoholic steatohepatitis in mice. J Clin Invest. 2012 Oct;122(10):3476-89. [Content Brief]

[2]. Karina Zitta, et al. Interleukin-1beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells. Biochem Biophys Res Commun. 2010 Sep 3;399(4):542-7. [Content Brief]

IL-1 beta Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Nano Res. 2026;19(1):94907900.

    Customer Validation
     ASC spot staining and IL-1β immunofluorescence staining of periodontal tissues from different groups.

  • 1/16

    IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Jan:290:127964. [Abstract]

    Customer Validation
     A histopathological evaluation was conducted on the kidneys of mice following their sacrifice two days after an intravenous injection with 1 × 106 C. albicans SC5314 cells, administered with or without 2 mg/kg CaHsp90 (n = 3). The kidneys were harvested, and two consecutive sections were stained using PAS stain and IHC staining with an anti-IL-1β antibody, respectively.

  • 1/16

    IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Jan:290:127964. [Abstract]

    Customer Validation
     J774A.1 cells were pre-incubated in the presence or absence of CaHsp90 at concentrations of 0.0625 μM, 0.25 μM, and 1 μM for 4 hours. Following this pre-incubation, the cells were exposed to C. albicans SC5314 cells (MOI = 10) for an additional 12 hours. Subsequently, the protein levels of IL-1β in the supernatant were analyzed using Western blotting.

  • 1/16

    IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Jul 17;353(Pt A):120310. [Abstract]

    Customer Validation
     Representative Western blot images of TNF-α, IL-6, and IL-1β.

  • 1/16

    IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15711-15737. [Abstract]

    Customer Validation
     The levels of IL-1β and caspase-1 in culture supernatants (SN), and NLRP3, pro-caspase-1, pro-IL-1β, and ASC in cells extracts (Input) of BMDMs cells were analyzed by Western blot.

  • IL-1 beta Antibody Product Image
    1/16
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • IL-1 beta Antibody Product Image
  • Experimental Validation Results for IL-1 beta Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-1 beta Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-1 beta Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-1 beta Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-1 beta Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody
  • Experimental Validation Results for IL-1 beta Antibody

  • IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Nano Res. 2026;19(1):94907900.

    ASC spot staining and IL-1β immunofluorescence staining of periodontal tissues from different groups.

  • IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Jan:290:127964. [Abstract]

    A histopathological evaluation was conducted on the kidneys of mice following their sacrifice two days after an intravenous injection with 1 × 106 C. albicans SC5314 cells, administered with or without 2 mg/kg CaHsp90 (n = 3). The kidneys were harvested, and two consecutive sections were stained using PAS stain and IHC staining with an anti-IL-1β antibody, respectively.

  • IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Jan:290:127964. [Abstract]

    J774A.1 cells were pre-incubated in the presence or absence of CaHsp90 at concentrations of 0.0625 μM, 0.25 μM, and 1 μM for 4 hours. Following this pre-incubation, the cells were exposed to C. albicans SC5314 cells (MOI = 10) for an additional 12 hours. Subsequently, the protein levels of IL-1β in the supernatant were analyzed using Western blotting.

  • IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Jul 17;353(Pt A):120310. [Abstract]

    Representative Western blot images of TNF-α, IL-6, and IL-1β.

  • IL-1 beta Antibody purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15711-15737. [Abstract]

    The levels of IL-1β and caspase-1 in culture supernatants (SN), and NLRP3, pro-caspase-1, pro-IL-1β, and ASC in cells extracts (Input) of BMDMs cells were analyzed by Western blot.

  • IL-1 beta Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IL-1 beta Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IL-1 beta Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IL-1 beta Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-1 beta Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-1 beta Antibody Product Image WB
  • IL-1 beta Antibody Product Image ICC
  • IL-1 beta Antibody Product Image ICC
  • IL-1 beta Antibody Product Image IHC-P
  • IL-1 beta Antibody Product Image IHC-P