IL-1 beta Antibody

Cat. No.: HY-P80720: Data Sheet COA
SDS
User Guide for Antibodies
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IL-1 beta Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 beta.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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IL-1 beta Antibody Featured Recommendations:

11 Publications Citing Use of MCE IL-1 beta Antibody

Histological Imaging/Staining

: IL-1 beta Antibody purchased from MCE. Usage Cited in: Nano Res. 2026;19(1):94907900.; ASC spot staining and IL-1β immunofluorescence staining of periodontal tissues from different groups.

: IL-1 beta Antibody purchased from MCE. Usage Cited in: Microbiol Res. 2024 Nov 5:290:127964. [Abstract]; A histopathological evaluation was conducted on the kidneys of mice following their sacrifice two days after an intravenous injection with 1 × 106 C. albicans SC5314 cells, administered with or without 2 mg/kg CaHsp90 (n = 3). The kidneys were harvested, and two consecutive sections were stained using PAS stain and IHC staining with an anti-IL-1β antibody, respectively.

: IL-1 beta Antibody purchased from MCE. Usage Cited in: Microbiol Res. 2024 Nov 5:290:127964. [Abstract]; J774A.1 cells were pre-incubated in the presence or absence of CaHsp90 at concentrations of 0.0625 μM, 0.25 μM, and 1 μM for 4 hours. Following this pre-incubation, the cells were exposed to C. albicans SC5314 cells (MOI = 10) for an additional 12 hours. Subsequently, the protein levels of IL-1β in the supernatant were analyzed using Western blotting.

: IL-1 beta Antibody purchased from MCE. Usage Cited in: J Ethnopharmacol. 2025 Jul 17;353(Pt A):120310. [Abstract]; Representative Western blot images of TNF-α, IL-6, and IL-1β.

: IL-1 beta Antibody purchased from MCE. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15711-15737. [Abstract]; The levels of IL-1β and caspase-1 in culture supernatants (SN), and NLRP3, pro-caspase-1, pro-IL-1β, and ASC in cells extracts (Input) of BMDMs cells were analyzed by Western blot.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

IL-1 beta Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-1 beta.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 31 kDa;

Observed band size: 17,31 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P01584

Gene ID

3553 [NCBI]

Immunogen

Synthetic peptide corresponding to Human IL1B AA range 181-230.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC IHC-P

Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of RAW 264.7 cells labeling IL-1 beta with IL-1 beta Antibody (HY-P80720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-1 beta Antibody (HY-P80720) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908)

Subcellular Localization：Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

Expression：
Tissue_specificity:Expression (protein level) in activated monocytes/macrophages.

Induction:By LPS (PubMed:15192144) . Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components in macrophages; EsxA is the most potent activator tested (at protein level) (PubMed:20148899) . In pancreatic islets, release is increased by high glucose treatment. In pancreatic islets and macrophages, release is also increased by endocannabinoid anandamide/AEA (PubMed:23955712)

Isoforms & Post-Translational Modification：
1) IL1beta (P01584): Number of amino acids: 269; Molecular weight (Da): 30,748
2) Mouse IL1beta (P10749): Number of amino acids: 269; Molecular weight (Da): 30,931
3) Rat IL1beta (a63264): Number of amino acids: 268; Molecular weight (Da): 30,644

Subunit：Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all (PubMed:17431422). Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling (PubMed:29030430). Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (PubMed:32272059). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059)

RRID

AB_3102173

Database

Entrez Gene: 3553 Human ; 16176 Mouse ;

SwissProt: P01584 Human ; P10749 Mouse ; Q63264 Rat

OMIM: 147720 Human

Research Field

Immunology

Synonyms

IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

IL-1 beta Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IL-1 beta Antibody

IL-1 beta Antibody Featured Recommendations:

11 Publications Citing Use of MCE IL-1 beta Antibody

Product Detail

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Background

Documentation

IL-1 beta Antibody Related Classifications

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