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Caspase-1 Antibody

(Synonyms: caspase-1 isoform alpha subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.)
8 Cited Publications
Customer Review

Based on 8 publication(s) in Google Scholar

Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat,

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Caspase-1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Caspase-1 Antibody

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8 Publications Citing Use of MCE Caspase-1 Antibody (5)
Flow CytometryIFWB
  • WB
  • Flow Cytometry
  • WB
  • IF
  • Flow Cytometry

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1μg/Test 1:100-500

Product Details

Description

Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat,
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3644161

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Caspase-1 Antibody
    Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human colon tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human testis tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Caspase-1 is the effector protease of canonical inflammasomes, where inflammasome activation enables processing and secretion of IL-1β and IL-18[1]. Mechanistically, caspase-1/11 cleaves gasdermin D (GSDMD), and GSDMD functions downstream as a pyroptosis executioner while also providing negative feedback on caspase-1/11 activation[2]. In endothelial models, homocysteine and lipopolysaccharide induced NLRP3 inflammasome assembly, caspase-1 activation, IL-1β cleavage, and caspase-1-dependent pyroptosis linked to endothelial dysfunction[3]. In respiratory infection models, Nontypeable Haemophilus influenzae activated caspase-1 and caspase-1 inhibition significantly reduced IL-1β release in respiratory cells and human lung tissue[4]. Compared with related isoforms, caspase-1 primarily controlled infected macrophage pyroptosis and IL-18 production in pneumonic melioidosis, whereas caspase-11 mediated infected lung epithelial-cell pyroptosis[5]. More broadly, murine caspase-11 and human caspase-4/5 define the noncanonical inflammasome pathway as cytosolic LPS sensors, separating their upstream recognition role from canonical procaspase-1 inflammasome activation[6]. For experimental applications, caspase-1 inhibitors can test IL-1β-dependent inflammatory output, while GSDMD-derived peptide inhibition provides a tool to suppress caspase-1/11 activation and downstream substrate cleavage[2][4].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (Caspase-1 subunit p20) and a 10 kDa (Caspase-1 subunit p10) subunit (PubMed:32109412).

  • SwissProt ID

    P29452

  • Gene ID
    834 [NCBI]
  • Synonyms

    caspase-1 isoform alpha subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.

References

[1]. Dinarello CA. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. doi: 10.1002/art.22841. PMID: 17763412. et al. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. [Content Brief]

[2]. Hu Y, et al. The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11. Proc Natl Acad Sci U S A. 2022 Nov 8;119(45):e2210809119. [Content Brief]

[3]. Xi H, et al. Caspase-1 Inflammasome Activation Mediates Homocysteine-Induced Pyrop-Apoptosis in Endothelial Cells. Circ Res. 2016 May 13;118(10):1525-39. [Content Brief]

[4]. detto Loria JR, et al. Nontypeable haemophilus influenzae (NTHi) leads to caspase-1-dependent upregulation of interleukin 1-beta (IL-1β) in respiratory cells and human lung tissue–A role of the “inflammasome” in respiratory tract infections. European Respiratory Journal. 2014 Jan 7;38(Suppl 55).

[5]. Wang J, et al. Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18. PLoS Pathog. 2018 May 23;14(5):e1007105. [Content Brief]

[6]. Wu J, et al. Pyroptosis by caspase-11 inflammasome-Gasdermin D pathway in autoimmune diseases. Pharmacol Res. 2021 Mar;165:105408. [Content Brief]

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Caspase-1 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Caspase-1 Antibody purchased from MedChemExpress. Usage Cited in: Nano Res. 2026;19(1):94907900.

    Customer Validation
     RAW264.7 cells were transfected with siNC or siTet1 for 48 h before stimulated or not with LPS. The indicated protein level was detected by western blot. Antibody: Caspase-1 Antibody (HY-P81232).

  • 1/16

    Caspase-1 Antibody purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jul:142:156698. [Abstract]

    Customer Validation
     Ophiopogonin C reduced pyroptosis macrophage in sepsis model of ALI Flow cytometry for pyroptosis cells. Antibody: Caspase-1 Antibody (1 h, HY-P81232).

  • 1/16

    Caspase-1 Antibody purchased from MedChemExpress. Usage Cited in: Neurochem Int. 2025 Sep:188:105994. [Abstract]

    Customer Validation
     Expression of ASC and Cleaved caspase-1 in KA-induced TLE model mice injected with NLRP6-AAV (n = 5). Primary antibodies: rabbit anti-ASC (1:1000), rabbit anti-Caspase-1 (1:1000; Caspase-1 Antibody (HY-P81232)), rabbit anti-GAPDH (1:5000).

  • 1/16

    Caspase-1 Antibody purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 Dec;32(6):327. [Abstract]

    Customer Validation
     Representative images of immunofluorescence staining of caspase-1 groups. Scale bar, 40 µm. Primary antibodies: Caspase-1 Antibody (1:500; 16 h, HY-P81232).

  • 1/16

    Caspase-1 Antibody purchased from MedChemExpress. Usage Cited in: Genes Immun. 2025 Aug;26(4):365-375. [Abstract]

    Customer Validation
     The percentage of pyroptotic cells (Caspase-1+/PI+ cells) was evaluated by flow cytometry. Primary antibody: Caspase-1 Antibody (incubate overnight at 4 °C, HY-P81232).

  • Caspase-1 Antibody Product Image
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  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Caspase-1 Antibody Product Image
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Experimental Validation Results for Caspase-1 Antibody
  • Caspase-1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Caspase-1 Antibody (HY-P81232, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase-1 Antibody Product Image WB
  • Caspase-1 Antibody Product Image IHC-P
  • Caspase-1 Antibody Product Image IHC-P
  • Caspase-1 Antibody Product Image IHC-P
  • Caspase-1 Antibody Product Image IHC-P
  • Caspase-1 Antibody Product Image IHC-P
  • Caspase-1 Antibody Product Image IHC-P