Caspase 1 Antibody

Cat. No.: HY-P80587: Data Sheet COA
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User Guide for Antibodies
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Caspase 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase 1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Caspase 1 Antibody Featured Recommendations:

5 Publications Citing Use of MCE Caspase 1 Antibody

: Caspase 1 Antibody purchased from MCE. Usage Cited in: J Transl Med. 2025 Jan 16;23(1):77. [Abstract]; Immunoblot analysis of pro-Caspase-1 and cleaved-caspase-1 in total lysates from liver tissue of Control, DDC-Fed, DDC-Withdrawn, and DDC-Refed mice.

: Caspase 1 Antibody purchased from MCE. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15711-15737. [Abstract]; The levels of IL-1β and caspase-1 in culture supernatants (SN), and NLRP3, pro-caspase-1, pro-IL-1β, and ASC in cells extracts (Input) of BMDMs cells were analyzed by Western blot.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Caspase 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase 1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P29466

Gene ID

834 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Caspase 1.AA range:342-391

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from HEK293 (lane 2(20μg) and HEK293(lane 3(40μg) using Caspase 1 (HY-P80587) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:15498465, PubMed:1574116, PubMed:26375003, PubMed:32051255, PubMed:37993714, PubMed:7876192, PubMed:9334240). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:15326478, PubMed:15498465, PubMed:1574116, PubMed:32051255, PubMed:7876192). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:15498465, PubMed:1574116, PubMed:7876192). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003). In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547); Apoptosis inactive; Apoptosis inactive

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:It is expressed at high levels in the spleen and lungs. It can also be detected in the liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidneys, and testes. Expression was not detected in brain tissue.

Induction:Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components; EsxA is the most potent activator tested (at protein level) (PubMed:20148899)

Isoforms & Post-Translational Modification：P29466 has 5 isomers: P29466-1: 45159 Da (predicted); P29466-2: 42888 Da (predicted); P29466-3: 35019 Da (predicted); P29466-4: 29821 Da (predicted); P29466-5: 10417 Da (predicted).
The two subunits are derived from the precursor sequence by an autocatalytic mechanism;Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8;Cleavage in the interdomain linker region is required to induce pyroptosis

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (Caspase-1 subunit p20) and a 10 kDa (Caspase-1 subunit p10) subunit (PubMed:32109412, PubMed:32553275, PubMed:8044845, PubMed:9987822). May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NLRP2 and whose function would be the activation of pro-inflammatory caspases (PubMed:15030775, PubMed:33420033). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity). Interacts with CARD8; interacts with the released C-terminus of CARD8 which forms an inflammasome and directly activates CASP1 to promote pyroptosis (PubMed:32051255). Both the p10 and p20 subunits interact with MEFV (PubMed:16785446). Interacts with CARD17P/INCA and CARD18 (PubMed:11051551, PubMed:15383541). Interacts with SERPINB1; this interaction regulates CASP1 activity (PubMed:30692621)

RRID

AB_3644160

Database

Entrez Gene: 834 Human ; 12362 Mouse

SwissProt: P29466 Human ; P29452 Mouse

OMIM: 147678 Human

Research Field

Cell Biology

Synonyms

CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Caspase 1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Caspase 1 Antibody

Caspase 1 Antibody Featured Recommendations:

5 Publications Citing Use of MCE Caspase 1 Antibody

Product Detail

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Background

Documentation

Caspase 1 Antibody Related Classifications

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