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Caspase 1 Antibody

(Synonyms: CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45)
5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

Caspase 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Caspase 1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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5 Publications Citing Use of MCE Caspase 1 Antibody (7)
WBIHC
  • WB
  • WB
  • WB
  • WB
  • IHC
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:50-1:100 1:10000

Product Details

Description

Caspase 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase 1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Caspase 1.AA range:342-391

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3644160

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Caspase 1 Antibody
    Western blot analysis of extracts from HEK293 (lane 2(20μg) and HEK293(lane 3(40μg) using Caspase 1 (HY-P80587) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase 1 Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase 1 Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Caspase-1 is the effector protease of canonical inflammasomes, where inflammasome activation enables processing and secretion of IL-1β and IL-18[1]. Mechanistically, caspase-1/11 cleaves gasdermin D (GSDMD), and GSDMD functions downstream as a pyroptosis executioner while also providing negative feedback on caspase-1/11 activation[2]. In endothelial models, homocysteine and lipopolysaccharide induced NLRP3 inflammasome assembly, caspase-1 activation, IL-1β cleavage, and caspase-1-dependent pyroptosis linked to endothelial dysfunction[3]. In respiratory infection models, Nontypeable Haemophilus influenzae activated caspase-1 and caspase-1 inhibition significantly reduced IL-1β release in respiratory cells and human lung tissue[4]. Compared with related isoforms, caspase-1 primarily controlled infected macrophage pyroptosis and IL-18 production in pneumonic melioidosis, whereas caspase-11 mediated infected lung epithelial-cell pyroptosis[5]. More broadly, murine caspase-11 and human caspase-4/5 define the noncanonical inflammasome pathway as cytosolic LPS sensors, separating their upstream recognition role from canonical procaspase-1 inflammasome activation[6]. For experimental applications, caspase-1 inhibitors can test IL-1β-dependent inflammatory output, while GSDMD-derived peptide inhibition provides a tool to suppress caspase-1/11 activation and downstream substrate cleavage[2][4].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:It is expressed at high levels in the spleen and lungs. It can also be detected in the liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidneys, and testes. Expression was not detected in brain tissue.

    Induction:Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components; EsxA is the most potent activator tested (at protein level) (PubMed:20148899)

  • Isoforms & Post-Translational Modification

    P29466 has 5 isomers: P29466-1: 45159 Da (predicted); P29466-2: 42888 Da (predicted); P29466-3: 35019 Da (predicted); P29466-4: 29821 Da (predicted); P29466-5: 10417 Da (predicted).
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism;Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8;Cleavage in the interdomain linker region is required to induce pyroptosis

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (Caspase-1 subunit p20) and a 10 kDa (Caspase-1 subunit p10) subunit (PubMed:32109412, PubMed:32553275, PubMed:8044845, PubMed:9987822). May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NLRP2 and whose function would be the activation of pro-inflammatory caspases (PubMed:15030775, PubMed:33420033). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity). Interacts with CARD8; interacts with the released C-terminus of CARD8 which forms an inflammasome and directly activates CASP1 to promote pyroptosis (PubMed:32051255). Both the p10 and p20 subunits interact with MEFV (PubMed:16785446). Interacts with CARD17P/INCA and CARD18 (PubMed:11051551, PubMed:15383541). Interacts with SERPINB1; this interaction regulates CASP1 activity (PubMed:30692621)

  • SwissProt ID

    P29466

  • Gene ID
    834 [NCBI]
  • Synonyms

    CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

  • Research Field

    Cell Biology

[1]. Dinarello CA. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. doi: 10.1002/art.22841. PMID: 17763412. et al. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. [Content Brief]

[2]. Hu Y, et al. The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11. Proc Natl Acad Sci U S A. 2022 Nov 8;119(45):e2210809119. [Content Brief]

[3]. Xi H, et al. Caspase-1 Inflammasome Activation Mediates Homocysteine-Induced Pyrop-Apoptosis in Endothelial Cells. Circ Res. 2016 May 13;118(10):1525-39. [Content Brief]

[4]. detto Loria JR, et al. Nontypeable haemophilus influenzae (NTHi) leads to caspase-1-dependent upregulation of interleukin 1-beta (IL-1β) in respiratory cells and human lung tissue–A role of the “inflammasome” in respiratory tract infections. European Respiratory Journal. 2014 Jan 7;38(Suppl 55).

[5]. Wang J, et al. Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18. PLoS Pathog. 2018 May 23;14(5):e1007105. [Content Brief]

[6]. Wu J, et al. Pyroptosis by caspase-11 inflammasome-Gasdermin D pathway in autoimmune diseases. Pharmacol Res. 2021 Mar;165:105408. [Content Brief]

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Caspase 1 Antibody Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg) and HEK293(lane 3(40μg) using Caspase 1 (HY-P80587) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 16;23(1):77. [Abstract]

    Customer Validation
     Immunoblot analysis of pro-Caspase-1 and cleaved-caspase-1 in total lysates from liver tissue of Control, DDC-Fed, DDC-Withdrawn, and DDC-Refed mice.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 16;23(1):77. [Abstract]

    Customer Validation
     In the co-culture system without LPS priming, Western blot analysis revealed increased protein levels of NLRP3 and pro-caspase-1 in macrophages.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15711-15737. [Abstract]

    Customer Validation
     The levels of IL-1β and caspase-1 in culture supernatants (SN), and NLRP3, pro-caspase-1, pro-IL-1β, and ASC in cells extracts (Input) of BMDMs cells were analyzed by Western blot.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jul 1;7(7):2110-2124. [Abstract]

    Customer Validation
     (A) Expression levels and (B−F) semiquantitative analysis of Hsp90α, Hsp90β, NLRP3, ASC, and caspase-1 in HFD-fed mice.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jul 1;7(7):2110-2124. [Abstract]

    Customer Validation
     Representative immunohistochemical images of the intensity of Hsp90α, Hsp90β, NLRP3, and caspase-1 in colon sections.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Nov 7;443(2):114325. [Abstract]

    Customer Validation
     The level of Caspase-1 Pro tended to decrease after MSC-Exos, pMSC-Exos, and MCC950 co-culture, and that of Caspase-1 p20, the active form of Caspase-1, decreased significantly after pMSC-Exos and MCC950 co-culture.

  • 1/16

    Caspase 1 Antibody purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Nov 7;443(2):114325. [Abstract]

    Customer Validation
     The expression of GSDMD-NT and Caspase-1 p20 increased significantly in the pMSC-Exos + PRDX-1-IN-1 group according to WB results in MSCs(mesenchymal stem cells).

  • Caspase 1 Antibody Product Image
    1/16
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Caspase 1 Antibody Product Image
  • Experimental Validation Results for Caspase 1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg) and HEK293(lane 3(40μg) using Caspase 1 (HY-P80587) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase 1 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase 1 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Experimental Validation Results for Caspase 1 Antibody
  • Caspase 1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg) and HEK293(lane 3(40μg) using Caspase 1 (HY-P80587) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Caspase 1 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase 1 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Caspase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80587, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Caspase 1 Antibody Product Image WB
  • Caspase 1 Antibody Product Image IHC-P
  • Caspase 1 Antibody Product Image IHC-P