Cleaved-Caspase-1 Antibody

Cat. No.: HY-P80622: Data Sheet COA
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Cleaved-Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cleaved-Caspase-1.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
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Cleaved-Caspase-1 Antibody Featured Recommendations:

16 Publications Citing Use of MCE Cleaved-Caspase-1 Antibody

IHC

: Cleaved-Caspase-1 Antibody purchased from MCE. Usage Cited in: J Transl Med. 2025 Jan 16;23(1):77. [Abstract]; Immunoblot analysis of pro-Caspase-1 and Cleaved-Caspase-1 in total lysates from liver tissue of Control, DDC-Fed, DDC-Withdrawn, and DDC-Refed mice.

: Cleaved-Caspase-1 Antibody purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48. [Abstract]; Lung tissue sections underwent immunohistochemistry (IHC) staining to assess the expression of ZDHHC21, Cleaved-Caspase-1 (c-Caspase-1), and the cleaved form of Gasdermin D (GSDMD-N) in Sham, CLP, and CLP + NLRP3/AIM2-IN-3 groups (scale bar = 100 μm).

: Cleaved-Caspase-1 Antibody purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48. [Abstract]; ZDHHC21, TBK1, p-TBK1, IRF3, p-IRF3, Cleaved-Caspase-1 (c-Caspase-1), and GSDMD-N protein expression were determined by Western blot analysis in control, LPS, and LPS + NLRP3/AIM2-IN-3 treated MLE-12 cells.

: Cleaved-Caspase-1 Antibody purchased from MCE. Usage Cited in: Inflammation. 2025 Mar 25. [Abstract]; Protein levels of N-GSDMD and Cleaved-Caspase 1 expression in BMDMs determined using WB analysis (n = 3).

: Cleaved-Caspase-1 Antibody purchased from MCE. Usage Cited in: Adv Mater. 2024 Sep 17:e2409066. [Abstract]; WB analysis of NLRP3, zDHHC5, zDHHC9, GSDMD-N and Cleaved Caspase-1 level after diverse treatments.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Cleaved-Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cleaved-Caspase-1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P29466

Gene ID

834 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Cleaved-Caspase 1.AA range:161-210

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using Cleaved-Caspase 1 Antibody (HY-P80622) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 1 Antibody (HY-P80622) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 1 Antibody (HY-P80622) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 1 Antibody (HY-P80622) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 1 Antibody (HY-P80622) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:15498465, PubMed:1574116, PubMed:26375003, PubMed:32051255, PubMed:37993714, PubMed:7876192, PubMed:9334240). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:15326478, PubMed:15498465, PubMed:1574116, PubMed:32051255, PubMed:7876192). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:15498465, PubMed:1574116, PubMed:7876192). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003). In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547); Apoptosis inactive; Apoptosis inactive

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:It is expressed at high levels in the spleen and lungs. It can also be detected in the liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidneys, and testes. Expression was not detected in brain tissue.

Induction:Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components; EsxA is the most potent activator tested (at protein level) (PubMed:20148899)

Isoforms & Post-Translational Modification：P29466 has 5 isomers: P29466-1: 45159 Da (predicted); P29466-2: 42888 Da (predicted); P29466-3: 35019 Da (predicted); P29466-4: 29821 Da (predicted); P29466-5: 10417 Da (predicted).
The two subunits are derived from the precursor sequence by an autocatalytic mechanism;Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8;Cleavage in the interdomain linker region is required to induce pyroptosis

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (Caspase-1 subunit p20) and a 10 kDa (Caspase-1 subunit p10) subunit (PubMed:32109412, PubMed:32553275, PubMed:8044845, PubMed:9987822). May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NLRP2 and whose function would be the activation of pro-inflammatory caspases (PubMed:15030775, PubMed:33420033). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity). Interacts with CARD8; interacts with the released C-terminus of CARD8 which forms an inflammasome and directly activates CASP1 to promote pyroptosis (PubMed:32051255). Both the p10 and p20 subunits interact with MEFV (PubMed:16785446). Interacts with CARD17P/INCA and CARD18 (PubMed:11051551, PubMed:15383541). Interacts with SERPINB1; this interaction regulates CASP1 activity (PubMed:30692621)

RRID

AB_3102146

Database

Entrez Gene: 834 Human ;

SwissProt: P29466 Human ;

OMIM: 147678 Human

Research Field

Cell Biology

Synonyms

CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Cleaved-Caspase-1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cleaved-Caspase-1 Antibody

Cleaved-Caspase-1 Antibody Featured Recommendations:

16 Publications Citing Use of MCE Cleaved-Caspase-1 Antibody

Product Detail

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Background

Documentation

Cleaved-Caspase-1 Antibody Related Classifications

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