Phospho-MLKL (Ser345) Antibody (YA174)

Cat. No.: HY-P80468: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-MLKL (Ser345) Antibody (YA174) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MLKL (Ser345).

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-MLKL (Ser345) Antibody (YA174) Featured Recommendations:

1 Publications Citing Use of MCE Phospho-MLKL (Ser345) Antibody (YA174)

•Br J Pharmacol. 2025 Aug 21. [Abstract]

: Phospho-MLKL (Ser345) Antibody (YA174) purchased from MCE. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]; Western blotting results of PELI1, RIP3, p-RIP3, RIP1, p-RIP1, MLKL and p-MLKL in different groups.

: Phospho-MLKL (Ser345) Antibody (YA174) purchased from MCE. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]; Immunofluorescence imaging of pancreatic tissue utilising dual labelling with DAPI for nuclear counterstaining, and an antibody specific to p-MLKL facilitates high-resolution visualisation of p-MLKL localisation.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Phospho-MLKL (Ser345) Antibody (YA174) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MLKL (Ser345).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

Q9D2Y4 Mouse

Gene ID

74568 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser345 of Mouse MLKL.AA range:314-363.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity)

Subcellular Localization：Cytoplasm; Cell membrane; Nucleus

Expression：
Tissue_specificity:It is highly expressed in the thymus, colon, intestine, liver, spleen, and lungs. It is expressed at lower levels in skeletal muscle, heart, and kidneys. It was not detected in brain tissue.

Isoforms & Post-Translational Modification：Q9D2Y4 has 2 isomers: Q9D2Y4-1: 54317 Da (predicted); Q9D2Y4-2: 53375 Da (predicted).
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:24012422, PubMed:24095729, PubMed:32200799). It also induces homotrimerization and localization to the plasma membrane (By similarity)

Subunit：Homooligomer (By similarity). Homotrimer; forms homotrimers on necroptosis induction (By similarity). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (By similarity). Within this complex, may play a role in the proper targeting of RIPK1-RIPK3 to its downstream effector PGAM5 (By similarity). Interacts with RIPK3; the interaction is direct and promotes its phosphorylation and subsequent activation (PubMed:22265413, PubMed:23612963, PubMed:24012422, PubMed:24095729, PubMed:27321907)

RRID

AB_3644175

Database

Entrez Gene: 74568 Mouse

SwissProt: Q9D2Y4 Mouse

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-MLKL (Ser345) Antibody (YA174) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-MLKL (Ser345) Antibody (YA174)

Phospho-MLKL (Ser345) Antibody (YA174) Featured Recommendations:

1 Publications Citing Use of MCE Phospho-MLKL (Ser345) Antibody (YA174)

Product Detail

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Background

Documentation

Phospho-MLKL (Ser345) Antibody (YA174) Related Classifications

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