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Phospho-MLKL (Ser345) Antibody (YA174)

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Based on 1 publication(s) in Google Scholar

Phospho-MLKL (Ser345) Antibody (YA174) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MLKL (Ser345).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Phospho-MLKL (Ser345) Antibody (YA174)

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1 Publications Citing Use of MCE Phospho-MLKL (Ser345) Antibody (YA174) (2)
WBIF
  • WB
  • IF

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500 1:50-1:100

Product Details

Description

Phospho-MLKL (Ser345) Antibody (YA174) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MLKL (Ser345).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

Entrez Gene: 74568 Mouse

SwissProt: Q9D2Y4 Mouse

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser345 of Mouse MLKL.AA range:314-363.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3644175

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    MLKL is a Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm; Cell membrane; Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the thymus, colon, intestine, liver, spleen, and lungs. It is expressed at lower levels in skeletal muscle, heart, and kidneys. It was not detected in brain tissue.

  • Isoforms & Post-Translational Modification

    Q9D2Y4 has 2 isomers: Q9D2Y4-1: 54317 Da (predicted); Q9D2Y4-2: 53375 Da (predicted).
    Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:24012422, PubMed:24095729, PubMed:32200799). It also induces homotrimerization and localization to the plasma membrane (By similarity)

  • Subunit

    Homooligomer (By similarity). Homotrimer; forms homotrimers on necroptosis induction (By similarity). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (By similarity). Within this complex, may play a role in the proper targeting of RIPK1-RIPK3 to its downstream effector PGAM5 (By similarity). Interacts with RIPK3; the interaction is direct and promotes its phosphorylation and subsequent activation (PubMed:22265413, PubMed:23612963, PubMed:24012422, PubMed:24095729, PubMed:27321907)

  • SwissProt ID

    Q9D2Y4 Mouse

  • Gene ID
    74568 [NCBI]
  • Research Field

    Signal Transduction

[1]. Wu J, et al. Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis. Cell Res. 2013 Aug;23(8):994-1006. [Content Brief]

[2]. Murphy JM, et al. The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity. 2013 Sep 19;39(3):443-53. [Content Brief]

[3]. Kaiser WJ, et al. Toll-like receptor 3-mediated necrosis via TRIF, RIP3, and MLKL. J Biol Chem. 2013 Oct 25;288(43):31268-79. [Content Brief]

[4]. Nogusa S, et al. RIPK3 Activates Parallel Pathways of MLKL-Driven Necroptosis and FADD-Mediated Apoptosis to Protect against Influenza A Virus. Cell Host Microbe. 2016 Jul 13;20(1):13-24. [Content Brief]

[5]. Zhang T, et al. Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis. Cell. 2020 Mar 19;180(6):1115-1129.e13. [Content Brief]

[6]. Jiao H, et al. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation. Nature. 2020 Apr;580(7803):391-395. [Content Brief]

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Phospho-MLKL (Ser345) Antibody (YA174) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Phospho-MLKL (Ser345) Antibody (YA174) purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]

    Customer Validation
     Western blotting results of PELI1, RIP3, p-RIP3, RIP1, p-RIP1, MLKL and p-MLKL in different groups.

  • 1/16

    Phospho-MLKL (Ser345) Antibody (YA174) purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]

    Customer Validation
     Immunofluorescence imaging of pancreatic tissue utilising dual labelling with DAPI for nuclear counterstaining, and an antibody specific to p-MLKL facilitates high-resolution visualisation of p-MLKL localisation.

  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image
    1/16
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)
  • Experimental Validation Results for Phospho-MLKL (Ser345) Antibody (YA174)

  • Phospho-MLKL (Ser345) Antibody (YA174) purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]

    Western blotting results of PELI1, RIP3, p-RIP3, RIP1, p-RIP1, MLKL and p-MLKL in different groups.

  • Phospho-MLKL (Ser345) Antibody (YA174) purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2025 Aug 21. [Abstract]

    Immunofluorescence imaging of pancreatic tissue utilising dual labelling with DAPI for nuclear counterstaining, and an antibody specific to p-MLKL facilitates high-resolution visualisation of p-MLKL localisation.

  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Phospho-MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image IHC-P
  • Phospho-MLKL (Ser345) Antibody (YA174) Product Image IHC-P