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Collagen I Antibody

(Synonyms: Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN.)
6 Cited Publications
Customer Review

Based on 6 publication(s) in Google Scholar

Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat, Rabbit

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Collagen I Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Collagen I Antibody

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6 Publications Citing Use of MCE Collagen I Antibody (5)
IFIHCWB
  • IF
  • IF
  • WB
  • IF
  • IHC

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-2000 1:100-500

Product Details

Description

Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit Predicted Reactivity: Chicken,Dog,Cow,Sheep
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 139 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 139 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human Collagen I: 1051-1150/1464

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103110

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen I Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Collagen I Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Collagen I is the predominant fibrillar collagen in the extracellular matrix and provides tensile strength, structural integrity, and mechanical support to bone, skin, tendon, ligament, and vascular tissues through the assembly of highly ordered collagen fibrils.[1][2] Type I collagen is composed of two α1 (I) chains and one α2 (I) chain that form a triple-helical procollagen molecule, which undergoes enzymatic processing and extracellular fibril formation to generate mature collagen fibers.[3][4] Mechanistically, collagen I regulates extracellular matrix organization and tissue architecture by forming stable cross-linked fibrillar networks that support cell adhesion, tissue repair, and connective tissue homeostasis.[1][3] In disease models, mutations affecting COL1A1 disrupt collagen assembly, fibrillogenesis, and matrix stability, leading to disorders including osteogenesis imperfecta and Ehlers-Danlos syndrome, highlighting the essential role of collagen I in skeletal and connective tissue integrity.[4] Compared with other collagen isoforms that form specialized extracellular matrix structures, collagen I is distinguished by its abundance and dominant contribution to fibrillar matrix formation in connective tissues.[1][2] For experimental applications, collagen I is widely used as a biomaterial and extracellular matrix model for investigating cell-matrix interactions, tissue remodeling, fibrosis, and regenerative processes because of its well-characterized fibrillar architecture and biomechanical properties.[1][2]

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:The fibers that make up tendons, ligaments, and bones. In bones, these fibers are mineralized by hydroxyapatite calcium.

  • Subunit

    Interacts with TRAM2 (PubMed:14749390).

  • SwissProt ID

    P02452

  • Gene ID
    1277 [NCBI]
  • Synonyms

    Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN.

[1]. Amirrah IN, et al. A Comprehensive Review on Collagen Type I Development of Biomaterials for Tissue Engineering: From Biosynthesis to Bioscaffold. Biomedicines. 2022 Sep 16;10(9):2307. [Content Brief]

[2]. Naomi R, et al. Current Insights into Collagen Type I. Polymers (Basel). 2021 Aug 9;13(16):2642. [Content Brief]

[3]. National Library of Medicine.

[4]. Collagen I gene information from NCBI.

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Collagen I Antibody Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Collagen I Antibody purchased from MedChemExpress. Usage Cited in: ACS Nano. 2026 Apr 21;20(15):11673-11691. [Abstract]

    Customer Validation
     Multiplex fluorescence staining of ECM components in tumor tissues, which were collected from breast cancer patients and orthotopic mouse models; Collagen I (COL1), green signals; TNC, aurantia signals; HA, red signals; scale bar, 200 μm. To detect the expression of typical ECM components, these sections were incubated with Collagen I Antibody (COL1, specific antibody, HY-P81227, 1:50 dilution), Tenascin-C (TNC, specific antibody, 1:100 dilution), and Hyaluronic acid (HA, specific antibody, 1:100 dilution), followed by the Multiplex Fluorescence Staining Kit based on Tyramide Signal Amplification (TSA) technology.

  • 1/16

    Collagen I Antibody purchased from MedChemExpress. Usage Cited in: PLoS One. 2026 May 26;21(5):e0350087. [Abstract]

    Customer Validation
     IF staining of α-SMA and Collagen I in PANC-1+PSCs spheroids before and after cryoablation. Nucleus were counterstained with DAPI. Different lowercase letters denote statistically significant differences within groups (p < 0.05). Scale bars = 100 μm.

  • 1/16

    Collagen I Antibody purchased from MedChemExpress. Usage Cited in: PLoS One. 2026 May 26;21(5):e0350087. [Abstract]

    Customer Validation
     WB analysis of TGF-β, Smad3, p-Smad3, α-SMA, and Collagen I in PANC-1+PSCs tumor spheroids under different treatment conditions: Control, cryo, and cryo with AG. Total protein extracted from spheroids and tissues was quantified via a BCA assay. Samples were resolved by SDS-PAGE and electroblotted onto PVDF membranes. Following blocking, membranes were probed overnight at 4°C with primary antibodies against TGF-β1, p-Smad3, Smad3, α-SMA, and Collagen I (HY-P81227), using GAPDH as the loading control. Bands were detected via ECL after incubation with HRP-linked secondary antibodies.

  • 1/16

    Collagen I Antibody purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 May 27;19(20):19126-19140. [Abstract]

    Customer Validation
     Collagen I (COL1) expression (green signals) in pancreatic tumors from patients and mouse models, scale bar, 100 μm. To identify COL1 expression, the tumor tissues from pancreatic cancer patients and mice models were respectively incubated with primary antibody against COL1 (Collagen I Antibody, HY-P81227, 1:2000) and secondary antibody, Alexa Fluor 647 Goat Anti-Rabbit lgG(H+L) (1:200), and counterstained with DAPI for CLSM imaging. The pseudo color of Alexa Fluor 647 was set to green.

  • 1/16

    Collagen I Antibody purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Aug 26:220:107933. [Abstract]

    Customer Validation
     IHC staining of fibrosis markers α-SMA, Collagen I, Vimentin and E-cadherin (n = 3). Scale bar: 100 μm.

  • Collagen I Antibody Product Image
    1/16
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody
  • Collagen I Antibody Product Image
  • Experimental Validation Results for Collagen I Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Collagen I Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Collagen I Antibody
  • Experimental Validation Results for Collagen I Antibody

  • Collagen I Antibody purchased from MedChemExpress. Usage Cited in: ACS Nano. 2026 Apr 21;20(15):11673-11691. [Abstract]

    Multiplex fluorescence staining of ECM components in tumor tissues, which were collected from breast cancer patients and orthotopic mouse models; Collagen I (COL1), green signals; TNC, aurantia signals; HA, red signals; scale bar, 200 μm. To detect the expression of typical ECM components, these sections were incubated with Collagen I Antibody (COL1, specific antibody, HY-P81227, 1:50 dilution), Tenascin-C (TNC, specific antibody, 1:100 dilution), and Hyaluronic acid (HA, specific antibody, 1:100 dilution), followed by the Multiplex Fluorescence Staining Kit based on Tyramide Signal Amplification (TSA) technology.

  • Collagen I Antibody purchased from MedChemExpress. Usage Cited in: PLoS One. 2026 May 26;21(5):e0350087. [Abstract]

    IF staining of α-SMA and Collagen I in PANC-1+PSCs spheroids before and after cryoablation. Nucleus were counterstained with DAPI. Different lowercase letters denote statistically significant differences within groups (p < 0.05). Scale bars = 100 μm.

  • Collagen I Antibody purchased from MedChemExpress. Usage Cited in: PLoS One. 2026 May 26;21(5):e0350087. [Abstract]

    WB analysis of TGF-β, Smad3, p-Smad3, α-SMA, and Collagen I in PANC-1+PSCs tumor spheroids under different treatment conditions: Control, cryo, and cryo with AG. Total protein extracted from spheroids and tissues was quantified via a BCA assay. Samples were resolved by SDS-PAGE and electroblotted onto PVDF membranes. Following blocking, membranes were probed overnight at 4°C with primary antibodies against TGF-β1, p-Smad3, Smad3, α-SMA, and Collagen I (HY-P81227), using GAPDH as the loading control. Bands were detected via ECL after incubation with HRP-linked secondary antibodies.

  • Collagen I Antibody purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 May 27;19(20):19126-19140. [Abstract]

    Collagen I (COL1) expression (green signals) in pancreatic tumors from patients and mouse models, scale bar, 100 μm. To identify COL1 expression, the tumor tissues from pancreatic cancer patients and mice models were respectively incubated with primary antibody against COL1 (Collagen I Antibody, HY-P81227, 1:2000) and secondary antibody, Alexa Fluor 647 Goat Anti-Rabbit lgG(H+L) (1:200), and counterstained with DAPI for CLSM imaging. The pseudo color of Alexa Fluor 647 was set to green.

  • Collagen I Antibody purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Aug 26:220:107933. [Abstract]

    IHC staining of fibrosis markers α-SMA, Collagen I, Vimentin and E-cadherin (n = 3). Scale bar: 100 μm.

  • Collagen I Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Collagen I Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Collagen I Antibody Product Image IHC-P
  • Collagen I Antibody Product Image IHC-P