2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies
  5. Collagen I Antibody

Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

    Collagen I Antibody purchased from MCE. Usage Cited in: Pharmacol Res. 2025 Aug 26:220:107933.  [Abstract]

    IHC staining of fibrosis markers α-SMA, Collagen I, Vimentin and E-cadherin (n = 3). Scale bar: 100 μm.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Verification Image

    • Background

    • Documentation

    Description

    Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.
    Host

    Rabbit
    Clonality

    Polyclonal
    Molecular Weight
    Predicted band size: 139 kDa;
    Observed band size: 139 kDa
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
    Species Reactivity
    Human, Mouse, Rat, Rabbit Predicted Reactivity: Chicken,Dog,Cow,Sheep
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
    SwissProt ID
    Gene ID
    Immunogen

    KLH conjugated synthetic peptide derived from human Collagen I: 1051-1150/1464
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		1:500-2000
    IHC-P
    IHC-P: Immunohistochemistry-Paraffin
    		1:100-500
    Sensitivity Endogenous Purity affinity purified
    Conjugation Non-conjugated Modification Unmodified
    Isotype IgG  
    Appearance

    Liquid
    Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Shipping

    Shipping with blue ice.
    Verification Image
    ALL WB IHC-P

    • Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

    • Western blot analysis of extracts from SK-N-SH (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) 、Mouse kidney (lane 4(20μg)) 、Mouse skin (lane 5(20μg)) and Rat skin (lane 6(20μg)) using COL1A1 Antibody (HY-P81227) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

    • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Background
    Function：Type I collagen is a member of group I collagen (fibrillar forming collagen)
    Subcellular Localization：Secreted, extracellular space, extracellular matrix
    Expression：
    Tissue_specificity:The fibers that make up tendons, ligaments, and bones. In bones, these fibers are mineralized by hydroxyapatite calcium.
    Subunit：Interacts with TRAM2 (PubMed:14749390).
    RRID
    Database

    Entrez Gene: 1277 Human ; 1278 Human ; 12842 Mouse ; 12843 Mouse ; 29393 Rat 84352

    SwissProt: P02452 Human ; P08123 Human ; P11087 Mouse ; Q01149 Mouse ; P02454 Rat P02466

    OMIM: 114000 Human

    Synonyms
    Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN.
    Documentation
    SDS (252 KB)

    COA (205 KB)

    User Guide for Antibodies (1077 KB)

    Collagen I Antibody Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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