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alpha smooth muscle Actin Antibody(YA3468)

(Synonyms: alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin; )
Customer Review

Based on 1 Customer Validation

alpha smooth muscle Actin Antibody(YA3468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha smooth muscle Actin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • alpha smooth muscle Actin Antibody(YA3468) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:1000-1:5000 1:1000-1:2000 1:1000-1:2000 1:100-1:2000 1:50-1:1000 1:2000-1:10000

Product Details

Description

alpha smooth muscle Actin Antibody(YA3468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha smooth muscle Actin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within N-terminal human alpha smooth muscle Actin.

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676650

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , C2C12(lane 3(20ug) and Neuro-2a(lane 4(20ug) using alpha smooth muscle Actin Antibody (HY-P83739) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling alpha smooth muscle Actin Antibody(YA3468) (HY-P83739 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    alpha smooth muscle Actin is an Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Induction:Up-regulated in response to enterovirus 71 (EV71) infection

    Positive sample: Saos-2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human breast carcinoma tissue, HeL

  • Subunit

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others

  • SwissProt ID

    P62736

  • Gene ID
    59 [NCBI]
  • Synonyms

    alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin;

alpha smooth muscle Actin Antibody(YA3468) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , C2C12(lane 3(20ug) and Neuro-2a(lane 4(20ug) using alpha smooth muscle Actin Antibody (HY-P83739) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling alpha smooth muscle Actin Antibody(YA3468) (HY-P83739 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • alpha smooth muscle Actin Antibody(YA3468) Product Image
    1/16
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • alpha smooth muscle Actin Antibody(YA3468) Product Image
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , C2C12(lane 3(20ug) and Neuro-2a(lane 4(20ug) using alpha smooth muscle Actin Antibody (HY-P83739) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling alpha smooth muscle Actin Antibody(YA3468) (HY-P83739 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • Experimental Validation Results for alpha smooth muscle Actin Antibody(YA3468)
  • alpha smooth muscle Actin Antibody(YA3468) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , C2C12(lane 3(20ug) and Neuro-2a(lane 4(20ug) using alpha smooth muscle Actin Antibody (HY-P83739) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • alpha smooth muscle Actin Antibody(YA3468) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

  • alpha smooth muscle Actin Antibody(YA3468) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

  • alpha smooth muscle Actin Antibody(YA3468) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling alpha smooth muscle Actin Antibody(YA3468) (HY-P83739 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • alpha smooth muscle Actin Antibody(YA3468) Product Image WB
  • alpha smooth muscle Actin Antibody(YA3468) Product Image IHC-P
  • alpha smooth muscle Actin Antibody(YA3468) Product Image IHC-P
  • alpha smooth muscle Actin Antibody(YA3468) Product Image FC