alpha smooth muscle Actin Antibody(YA3468)

Cat. No.: HY-P83739: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

alpha smooth muscle Actin Antibody(YA3468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha smooth muscle Actin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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alpha smooth muscle Actin Antibody(YA3468) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

alpha smooth muscle Actin Antibody(YA3468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha smooth muscle Actin.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P62736

Gene ID

59 [NCBI]

Immunogen

Synthetic peptide within N-terminal human alpha smooth muscle Actin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:50000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:2000-1:3000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50000-1:100000
FC FC: Flow Cytometry	1:1000-1:2000
mIHC mIHC: Multiplex Immunohistochemical	1:2000-1:10000

Purity	Protein A affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB IHC-P FC

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , C2C12(lane 3(20ug) and Neuro-2a(lane 4(20ug) using alpha smooth muscle Actin Antibody (HY-P83739) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using alpha smooth muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
Flow cytometric analysis of 1X10^6 HepG2 cells labeling alpha smooth muscle Actin Antibody(YA3468) (HY-P83739 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Induction:Up-regulated in response to enterovirus 71 (EV71) infection

Positive sample: Saos-2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human breast carcinoma tissue, HeL

Subunit：Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others

RRID

AB_3676650

Database

Entrez Gene: 59 Human ; 11475 Mouse ; 81633 Rat

SwissProt: P62736 Human ; P62737 Mouse ; P62738 Rat

OMIM: 611788 Human

Synonyms

alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin;

Documentation

Select Batch:

Data Sheet (234 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

alpha smooth muscle Actin Antibody(YA3468) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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alpha smooth muscle Actin Antibody(YA3468)

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