1. Cell Cycle/DNA Damage
  2. DNA Stain
  3. DAPI dihydrochloride

DAPI dihydrochloride  (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)

Cat. No.: HY-D0814 Purity: 99.27%
COA Handling Instructions

DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells.

For research use only. We do not sell to patients.

DAPI dihydrochloride Chemical Structure

DAPI dihydrochloride Chemical Structure

CAS No. : 28718-90-3

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 51 In-stock
Solution
10 mM * 1 mL in DMSO USD 51 In-stock
Solid
1 mg USD 30 In-stock
5 mg USD 46 In-stock
10 mg USD 78 In-stock
25 mg USD 163 In-stock
50 mg USD 260 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 40 publication(s) in Google Scholar

Other Forms of DAPI dihydrochloride:

Top Publications Citing Use of Products

36 Publications Citing Use of MCE DAPI dihydrochloride

IF

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123.  [Abstract]

    Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952.  [Abstract]

    Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15;96:468-479.  [Abstract]

    CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146.  [Abstract]

    MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1].

    In Vitro

    General Protocol
    1. Preparation of DAPI working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg DAPI in 1 mL ddH2O to obtain 1 mg/mL of stock solution.
    Note: It is recommended to store the stock solution at -20 °C or -80 °C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of DAPI working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution.
    Note: Please adjust the concentration of DAPI working solution according to the actual situation.
    2. Cell staining
    2.1 Suspension cells(6-well plate)
    a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
    c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    d. Wash twice with PBS, 5 minutes each time.
    e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a. Culture adherent cells on sterile coverslips.
    b. Remove the coverslip from the medium and aspirate excess medium.
    c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
    d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

    Storage
    -20°C, 1 year. Protect from light

    Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation.
    2. This product is for R&D use only, not for drug, household, or other uses.
    3. For your safety and health, please wear a lab coat and disposable gloves to operate.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    350.25

    Formula

    C16H17Cl2N5

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    Emission (Em)

    451

    Excitation (Ex)

    356

    SMILES

    N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 75 mg/mL (214.13 mM; Need ultrasonic)

    H2O : 5.56 mg/mL (15.87 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8551 mL 14.2755 mL 28.5510 mL
    5 mM 0.5710 mL 2.8551 mL 5.7102 mL
    10 mM 0.2855 mL 1.4276 mL 2.8551 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.27%

    Dyeing Example
    References
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    DAPI dihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    DAPI dihydrochloride
    Cat. No.:
    HY-D0814
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