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DAPI dihydrochloride

(Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)
169 Cited Publications
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Based on 169 publication(s) in Google Scholar

DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).

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  • Reinheit: 99.90%
  • CAS. Nr.: 28718-90-3
  • Formel: C16H17Cl2N5
  • Molecular Weight:350.25
  • Speicherung:

    -20°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • DAPI dihydrochloride Chemical Structure
    DAPI dihydrochloride Chemical Structure
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
  • Experimental Validation Results for DAPI dihydrochloride
CAS. Nr.: 28718-90-3
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  • DAPI dihydrochloride Chemical Structure
    DAPI dihydrochloride Chemical Structure
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    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]

    Customer Validation
     Effects of silencing and overexpression of vimentin on apoptosis of MH7A cells. TMRM/DAPI staining (n = 4).

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2022 Jun 6;15(6):721. [Abstract]

    Customer Validation
     The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123. [Abstract]

    Customer Validation
     Mice intestine tissue sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections were stained with 0.1 μg/mL DAPI.

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar:68:153146. [Abstract]

    Customer Validation
     MC3T3-E1 cells incubated with 1 μg/mL DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively. Scale bar = 20 µm.

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar:68:153146. [Abstract]

    Customer Validation
     MC3T3-E1 cells incubated with 1 μg/mL DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively. Scale bar = 20 µm.

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Onco Targets Ther. 2020 Oct 6:13:9941-9952. [Abstract]

    Customer Validation
     Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

  • 1/16

    DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15:96:468-479. [Abstract]

    Customer Validation
     CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

  • DAPI dihydrochloride