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  2. DAPI dihydrochloride

DAPI dihydrochloride (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride)

Cat. No.: HY-D0814 Purity: >98.0%
Handling Instructions

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

For research use only. We do not sell to patients.

DAPI dihydrochloride Chemical Structure

DAPI dihydrochloride Chemical Structure

CAS No. : 28718-90-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 78 In-stock
Estimated Time of Arrival: December 31
25 mg USD 156 In-stock
Estimated Time of Arrival: December 31
50 mg USD 276 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

In Vitro

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].

Molecular Weight

350.25

Formula

C₁₆H₁₇Cl₂N₅

CAS No.

28718-90-3

SMILES

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 75 mg/mL (214.13 mM; Need ultrasonic)

H2O : 5 mg/mL (14.28 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8551 mL 14.2755 mL 28.5510 mL
5 mM 0.5710 mL 2.8551 mL 5.7102 mL
10 mM 0.2855 mL 1.4276 mL 2.8551 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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Keywords:

DAPI4',6-Diamidino-2-phenylindoleOthersInhibitorinhibitorinhibit

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Product name:
DAPI dihydrochloride
Cat. No.:
HY-D0814
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