MitoTracker Deep Red FM
Based on 14 publication(s) in Google Scholar
MitoTracker Deep Red (MTDR) FM fluorescent dye that selectively accumulates in the mitochondrial matrix. MitoTracker Deep Red FM covalently binds mitochondrial proteins by reacting with free mercaptan of cysteine residues, allowing staining of mitochondrial membrane potential independent of membrane potential. Excitation/emission wavelength 644/665 nm. MitoTracker Deep Red dyes have an excitation/emission wavelength of 633/650-750 nm.
The Ex/Em of MitoTracker Deep Red (MTDR) FM is 644/665 nm.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 873315-86-7
- Formula: C34H36Cl2N2
- Molecular Weight:543.57
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MitoTracker Deep Red FM
More- ACS Nano. 2025 Jan 21;19(2):2695-2714. [Abstract]
- Autophagy. 2024 Nov;20(11):2556-2570. [Abstract]
- J Nanobiotechnology. 2025 Jul 4;23(1):485. [Abstract]
- J Hazard Mater. 2025 Mar 18:492:137969. [Abstract]
- Mater Today Bio. 2025 Oct 30:35:102483. [Abstract]
- ACS Appl Mater Interfaces. 2025 Mar 5;17(9):13461-13470. [Abstract]
- NanoImpact. 2025 Jun 15:39:100572. [Abstract]
- Int Immunopharmacol. 2025 Oct 25;167:115707.
- FASEB J. 2026 Mar 31;40(6):e71682. [Abstract]
- Toxicol Appl Pharmacol. 2025 May:498:117284. [Abstract]
- J Photochem Photobiol. 2025 Dec 20.
- bioRxiv. 2025 Oct 22.
- bioRxiv. 2025 Sep 17.
- bioRxiv. 2025 Sep 4:2025.09.02.671886. [Abstract]
Customer Validation & Images
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IF
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Flow Cytometry
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IF
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IF
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Biological Activity
The Ex/Em of MitoTracker Deep Red (MTDR) FM is 644/665 nm.
Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of MitoTracker Deep Red FM working solution
1.1 Preparation of storage solution
Prepare a 1 mM solution of MitoTracker Deep Red FM using DMSO.
1.2 Preparation of working solution
Use pre-heated serum-free cell culture medium or PBS to dilute the storage solution at a ratio of 1:5000 - 1:50000, and prepare a 20 - 200 nM MitoTracker Deep Red FM working solution.
Note: Please adjust the concentration of MitoTracker Deep Red FM working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to collect the cells, then add PBS for two washes, each for 5 minutes. The cell density is 1×106 per mL.
2.2 Add 1 mL of MitoTracker Deep Red FM solution and incubate at room temperature for 15 to 45 minutes.
2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cytological staining (adherent cells) 3.1 Cultivate adherent cells on a sterile cover glass.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 15 to 45 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Then observe using a fluorescence microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 873315-86-7
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Appearance Solid
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Molecular Weight 543.57
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Formula C34H36Cl2N2
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Color Blue to dark blue
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SMILES
ClCC1=CC=C(CN2C3=C(C(C)(C)/C2=C/C=C/C=C/C4=[N+](C)C5=C(C4(C)C)C=CC=C5)C=CC=C3)C=C1.[Cl-]
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Synonyms
MTDR FM
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (14)
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Journal Impact Factor
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Most Recent
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ACS Nano
2025 Jan 21;19(2):2695-2714. PMID: 39787443 -
Autophagy
Signal-sustained imaging of mitophagy with an Enzyme-Activatable Metabolic Lipid-Labeling Probe. [Abstract]2024 Nov;20(11):2556-2570. PMID: 38873937
MitoTracker Deep Red FM purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Nov;20(11):2556-2570. [Abstract]
Colocalization of AzRed Mito-proGreen with MitoTracker Deep Red. AzRed+ Mito-proGreen+ HeLa cells were stained with MitoTracker Deep Red (0.2 μM, 15 min) and then imaged by confocal microscopy. Scale bars: 10 μm.
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J Nanobiotechnology
SIRT5-modified human umbilical cord mesenchymal stem cells loaded with antioxidant polydopamine nanozyme enhance parpi resistance in ovarian cancer via fatty acid metabolism reprogramming. [Abstract]2025 Jul 4;23(1):485. PMID: 40616128 -
J Hazard Mater
Isobavachalcone confers protection against Cryptococcus neoformans-induced ferroptosis in Caenorhabditis elegans via lifespan extension and GSH-GPX-1 axis modulation. [Abstract]2025 Mar 18:492:137969. PMID: 40154123 -
Mater Today Bio
ATG7-driven mitophagy in BMSC@CS hydrogel reprograms metabolism to boost bone regeneration. [Abstract]2025 Oct 30:35:102483. PMID: 41281656
MitoTracker Deep Red FM purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Oct 30:35:102483. [Abstract]
MSCs were incubated with mitochondrial tracker (1 mM) for 30 min at 37 °C in the dark following 48 h treatment. Fluorescence signals were observed by fluorescence microscopy.
MitoTracker Deep Red FM purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Oct 30:35:102483. [Abstract]
MSCs were incubated with mitochondrial tracker (1 mM) for 30 min at 37 °C in the dark following 48 h treatment. Fluorescence signals were quantified by flow cytometry.
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ACS Appl Mater Interfaces
FRET Nanosensor Based on DNA Tetrahedron for Visualizing PLD3 Fluctuation in Mouse Models of Alzheimer's Disease. [Abstract]2025 Mar 5;17(9):13461-13470. PMID: 39973147 -
NanoImpact
CdTe quantum dots induce apoptosis in RSC96 cells by disrupting calcium homeostasis and triggering subcellular structural dysfunction. [Abstract]2025 Jun 15:39:100572. PMID: 40527434
MitoTracker Deep Red FM purchased from MedChemExpress. Usage Cited in: NanoImpact. 2025 Jun 15:39:100572. [Abstract]
Mitochondria in RSC96 cells from control and 80 μM CdTe QDs exposure groups were labeled by Mito-Tracker Red probe (500 nM, 37 °C for a period of 30 min), scale bar = 20 μm.
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FASEB J
Diminazene Aceturate Ameliorates Hypertension-Induced Cognitive Impairment by Disrupting the CCN1-Integrin αvβ6-TGF-β Axis and Preserving Mitochondrial Integrity. [Abstract]2026 Mar 31;40(6):e71682. PMID: 41860098 -
Toxicol Appl Pharmacol
The resveratrol attenuates reactive oxygen species mediated DNA damage in cardiac malformations caused by 4-tert-octylphenol. [Abstract]2025 May:498:117284. PMID: 40023230 -
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MitoTracker Deep Red FM purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Oct 22.
LCSM images of cardiac cells after the uptake of Mito@LLPS hydrogel. Isolated mitochondria were visualized using MitoTracker Deep Red FM (100 nmol/L). Scale bars, top: 50 μm, bottom: 20 μm.
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bioRxiv
ACTL6A regulates the Warburg effect through coordinated activation of AP-1 signaling in head and neck squamous cell carcinoma. [Abstract]2025 Sep 4:2025.09.02.671886. PMID: 40950224
Solvent & Solubility
DMSO : 125 mg/mL (229.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Buckman JF, et al. MitoTracker labeling in primary neuronal and astrocytic cultures: influence of mitochondrial membrane potential and oxidants. J Neurosci Methods. 2001 Jan 15;104(2):165-76. [Content Brief]
[2]. Wang FX, et al. Mitochondria-Accumulating Rhenium(I) Tricarbonyl Complexes Induce Cell Death via Irreversible Oxidative Stress and Glutathione Metabolism Disturbance. ACS Appl Mater Interfaces. 2019 Apr 10;11(14):13123-13133. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8397 mL | 9.1984 mL | 18.3969 mL | 45.9922 mL |
| 5 mM | 0.3679 mL | 1.8397 mL | 3.6794 mL | 9.1984 mL | |
| 10 mM | 0.1840 mL | 0.9198 mL | 1.8397 mL | 4.5992 mL | |
| 15 mM | 0.1226 mL | 0.6132 mL | 1.2265 mL | 3.0661 mL | |
| 20 mM | 0.0920 mL | 0.4599 mL | 0.9198 mL | 2.2996 mL | |
| 25 mM | 0.0736 mL | 0.3679 mL | 0.7359 mL | 1.8397 mL | |
| 30 mM | 0.0613 mL | 0.3066 mL | 0.6132 mL | 1.5331 mL | |
| 40 mM | 0.0460 mL | 0.2300 mL | 0.4599 mL | 1.1498 mL | |
| 50 mM | 0.0368 mL | 0.1840 mL | 0.3679 mL | 0.9198 mL | |
| 60 mM | 0.0307 mL | 0.1533 mL | 0.3066 mL | 0.7665 mL | |
| 80 mM | 0.0230 mL | 0.1150 mL | 0.2300 mL | 0.5749 mL | |
| 100 mM | 0.0184 mL | 0.0920 mL | 0.1840 mL | 0.4599 mL |
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