MitoTracker Deep Red FM (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of MitoTracker Deep Red FM working solution
Dilute the stock solution in serum-free cell culture medium or PBS dilute.
Note: Please adjust the concentration of MitoTracker Deep Red FM working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 15-45 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
4. Precautions
4.1 Please adjust the concentration of MitoTracker Deep Red FM working solution according to the actual situation.
4.2 This product is for R&D use only, not for drug, household, or other uses.
4.3 For your safety and health, please wear a lab coat and disposable gloves to operate.
4.4 It is recommended to aliquot the MitoTracker Deep Red FM stock solution and store it at -20 °C or -80 °C away from light, avoiding repeated freeze-thaw cycles. It can be stored for 1 month at -20 °C and for half a year at -80 °C.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.