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RUNX2 Antibody (YA085)

(Synonyms: AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A)
6 Cited Publications
Customer Review

Based on 6 publication(s) in Google Scholar

RUNX2 Antibody (YA085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • RUNX2 Antibody (YA085) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:5000-1:10000 1:1000-1:5000 1:200-1:500 1:200-1:1000 1:5000 1-2 μg/sample 1:2000

Product Details

Description

RUNX2 Antibody (YA085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human RUNX2.AA range:300-450.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102423

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RUNX2 Antibody (YA085)
    Western blot analysis of extracts from NIH3T3 (lane 2(20μg) , MDA-MB231(lane 3(20μg) and Saos-2(lane 4(20μg) using RUNX2(HY-P80316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    RUNX2 (Runt-related transcription factor 2) is a master transcription factor that governs osteoblast differentiation, bone formation, and skeletal development by regulating osteogenic gene expression programs[1][2]. RUNX2 functions early during mesenchymal stem cell commitment to the osteoblast lineage and remains essential for progression through multiple stages of osteogenesis, while its expression is tightly controlled during osteoblast maturation[3][4]. Mechanistically, RUNX2 integrates signals from developmental and intracellular pathways, including MAPK-mediated activation and BMP-2/SMAD signaling, to coordinate transcriptional programs required for bone matrix production and mineralization[2][5]. RUNX2 also participates in chondrocyte maturation and endochondral bone development, highlighting its central role in skeletal tissue formation beyond osteoblast biology[3][6]. In disease and experimental models, loss of RUNX2 function causes profound skeletal abnormalities, and RUNX2 deficiency in mice results in the absence of osteoblasts and impaired bone formation, establishing RUNX2 as an indispensable regulator of skeletal development[3][7]. In humans, heterozygous mutations in RUNX2 cause cleidocranial dysplasia, a hereditary skeletal disorder characterized by defects in bone development[7][8]. Compared with related RUNX family members, RUNX2 exhibits a specialized role in osteoblast lineage specification and bone-forming activity, although partial functional overlap with RUNX3 has been reported during chondrocyte maturation[3]. Two major RUNX2 isoforms have been described, with evidence indicating distinct contributions to osteoblast development, where type I is associated with early osteoblastogenesis and type II contributes to later stages of osteoblastic maturation[9]. For experimental applications, RUNX2 activity is widely used as a molecular indicator of osteogenic differentiation, and modulation of BMP-2 signaling can alter RUNX2 expression in osteoblast models, providing a useful framework for studying bone formation mechanisms[5].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Specifically expressed in osteoblasts

  • Subunit

    Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with HIVEP3. Interacts with IFI204. Interaction with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors. Binds to HIPK3. Interacts with FOXO1 (via a C-terminal region); the interaction inhibits RUNX2 transcriptional activity towards BGLAP. This interaction is prevented on insulin or IGF1 stimulation as FOXO1 is exported from the nucleus (By similarity). Interacts with CCNB1, KAT6A and KAT6B. Interacts with FOXP3. Interacts with TMEM119 (By similarity). Interacts with OLFM2 (By similarity). Interacts with IPO7; the interaction inhibits RUNX2 nuclear translocation in osteoblasts (By similarity)

  • SwissProt ID

    Q13950

  • Gene ID
    860 [NCBI]
  • Synonyms

    AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Vimalraj S, et al. Runx2: Structure, function, and phosphorylation in osteoblast differentiation. Int J Biol Macromol. 2015;78:202-8. [Content Brief]

[2]. Franceschi RT, et al. Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways. J Cell Biochem. 2003 Feb 15;88(3):446-54. [Content Brief]

[3]. Komori T. Regulation of Proliferation, et al. Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx2. Int J Mol Sci. 2019 Apr 4;20(7):1694. [Content Brief]

[4]. Zhu S, et al. Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis. Cell Discov. 2024 Jul 2;10(1):71. [Content Brief]

[5]. Young-Eun Cho, et al. Zinc upregulates bone-specific transcription factor Runx2 expression via BMP-2 signaling and Smad-1 phosphorylation in osteoblasts. J Nutr Health. 2018 Feb;51(1):23-30.

[6]. Liu TM, et al. Transcriptional regulatory cascades in Runx2-dependent bone development. Tissue Eng Part B Rev. 2013 Jun;19(3):254-63. [Content Brief]

[7]. Baniwal SK, et al. Runx2 promotes both osteoblastogenesis and novel osteoclastogenic signals in ST2 mesenchymal progenitor cells. Osteoporos Int. 2012 Apr;23(4):1399-413. [Content Brief]

[8]. RUNX2 gene information from NCBI.

[9]. Bruderer M, et al. Role and regulation of RUNX2 in osteogenesis. Eur Cell Mater. 2014 Oct 23;28:269-86. [Content Brief]

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RUNX2 Antibody (YA085) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 2(20μg) , MDA-MB231(lane 3(20μg) and Saos-2(lane 4(20μg) using RUNX2(HY-P80316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RUNX2 Antibody (YA085) Product Image
    1/16
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • RUNX2 Antibody (YA085) Product Image
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 2(20μg) , MDA-MB231(lane 3(20μg) and Saos-2(lane 4(20μg) using RUNX2(HY-P80316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RUNX2 Antibody (YA085)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • Experimental Validation Results for RUNX2 Antibody (YA085)
  • RUNX2 Antibody (YA085) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH3T3 (lane 2(20μg) , MDA-MB231(lane 3(20μg) and Saos-2(lane 4(20μg) using RUNX2(HY-P80316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RUNX2 Antibody (YA085) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • RUNX2 Antibody (YA085) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using RUNX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80316, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • RUNX2 Antibody (YA085) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RUNX2 Antibody (YA085) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling RUNX2 with RUNX2 Antibody (HY-P80316) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RUNX2 Antibody (HY-P80316) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RUNX2 Antibody (YA085) Product Image WB
  • RUNX2 Antibody (YA085) Product Image IHC-P
  • RUNX2 Antibody (YA085) Product Image IHC-P
  • RUNX2 Antibody (YA085) Product Image ICC
  • RUNX2 Antibody (YA085) Product Image ICC