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SP7/Osterix Antibody (YA3539)

(Synonyms: MGC126598 antibody; Osterix antibody; Osx antibody; Sp 7 antibody; SP7 antibody; Sp7 transcription factor antibody; SP7_HUMAN antibody; Transcription factor Sp7 antibody; Zinc finger protein osterix antibody; )
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Based on 1 publication(s) in Google Scholar

SP7/Osterix Antibody (YA3539) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SP7/Osterix.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • SP7/Osterix Antibody (YA3539) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:2000 1:200-1:1000 1:500 1:200

Product Details

Description

SP7/Osterix Antibody (YA3539) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SP7/Osterix.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

Recombinant protein within human Sp7 aa 1-300.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103226

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    Western blot analysis of extracts from Saos-2 (lane 2(20μg) , Saos-2 (lane 3(40μg) ,using SP7/Osterix Antibody (HY-P81125). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    SP7/Osterix transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences[1].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Restricted to bone-derived cell
    Positive sample: Saos-2 cell lysates, human osteosarcoma tissue, mouse embryo tissue, rat embryo tissue, E14.5 mouse embryo tissue.

  • Isoforms & Post-Translational Modification

    Q8TDD2 has 2 isomers: Q8TDD2-1: 44994 Da (predicted); Q8TDD2-2: 43092 Da (predicted).
    Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours;Propionylated. Depropionylation at Lys-371 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts

  • Subunit

    Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity

  • SwissProt ID

    Q8TDD2

  • Gene ID
    121340 [NCBI]
  • Synonyms

    MGC126598 antibody; Osterix antibody; Osx antibody; Sp 7 antibody; SP7 antibody; Sp7 transcription factor antibody; SP7_HUMAN antibody; Transcription factor Sp7 antibody; Zinc finger protein osterix antibody;

SP7/Osterix Antibody (YA3539) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane 2(20μg) , Saos-2 (lane 3(40μg) ,using SP7/Osterix Antibody (HY-P81125). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SP7/Osterix Antibody (YA3539) Product Image
    1/16
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • SP7/Osterix Antibody (YA3539) Product Image
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane 2(20μg) , Saos-2 (lane 3(40μg) ,using SP7/Osterix Antibody (HY-P81125). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • Experimental Validation Results for SP7/Osterix Antibody (YA3539)
  • SP7/Osterix Antibody (YA3539) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Saos-2 (lane 2(20μg) , Saos-2 (lane 3(40μg) ,using SP7/Osterix Antibody (HY-P81125). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SP7/Osterix Antibody (YA3539) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SP7/Osterix Antibody (YA3539) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SP7/Osterix Antibody (YA3539) Product Image WB
  • SP7/Osterix Antibody (YA3539) Product Image IHC-P
  • SP7/Osterix Antibody (YA3539) Product Image IHC-P