Collagen III Antibody

Cat. No.: HY-P81101: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Collagen III Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen III.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Collagen III Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Collagen III Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen III.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 117kDa;

Observed band size: 139-150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Dog, Rabbit Predicted Reactivity: Mouse, Rat, Chicken, Cow

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P02461

Gene ID

1281 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain: 1301-1400/1466

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Isoforms & Post-Translational Modification：P02461 has 2 isomers: P02461-1: 138564 Da (predicted); P02461-2: 111907 Da (predicted).
Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains;O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group

Subunit：Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines. Interacts with ADGRG1 (PubMed:28258187)

RRID

AB_3103059

Database

Entrez Gene: 1281 Human ;

SwissProt: P02461 Human ;

OMIM: 130050 Human

Synonyms

COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen; CO3A1_HUMAN; Collagen alpha-1(III) chain; Type III collagen; type III preprocollagen alpha 1 chain.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Collagen III Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Collagen III Antibody

Collagen III Antibody Featured Recommendations:

Product Detail

Background

Documentation

Collagen III Antibody Related Classifications

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