Collagen II Antibody

Cat. No.: HY-P81047: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Collagen II Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen II.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Collagen II Antibody Featured Recommendations:

1 Publications Citing Use of MCE Collagen II Antibody

•Adv Mater. 2025 Apr;37(17):e2416349. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Collagen II Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen II.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 117 kDa;

Observed band size: 120-230 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse,Rat,Rabbit,Chicken,Pig,Cow,Dog,Guinea Pig

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P02458

Gene ID

1280 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Collagen II 1201-1300/1487

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Purity	Affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
2.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:Isomer 2 is highly expressed in juvenile chondrocytes and lowly expressed in fetal chondrocytes.

Isoforms & Post-Translational Modification：P02458 has 3 isomers: P02458-2: 141785 Da (predicted); P02458-1: 134389 Da (predicted); P02458-3: 29781 Da (predicted).
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2;Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains;Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline;Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains;O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide

Subunit：Homotrimers of alpha 1(II) chains

RRID

AB_3103040

Database

Entrez Gene: 1280 Human ; 12824 Mouse ; 25412 Rat

SwissProt: P02458 Human ; P28481 Mouse ; P05539 Rat

OMIM: 183900 Human

Synonyms

Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; Alpha-1 type II collagen; CO2A1_HUMAN.; CollagenII;

Documentation

Select Batch:

Data Sheet (233 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Collagen II Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Collagen II Antibody

Collagen II Antibody Featured Recommendations:

1 Publications Citing Use of MCE Collagen II Antibody

Product Detail

Background

Documentation

Collagen II Antibody Related Classifications

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