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Collagen II Antibody

(Synonyms: Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; Alpha-1 type II collagen; CO2A1_HUMAN.; CollagenII; )
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Collagen II Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen II.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue

  • Reactivity :

    Human

  • Formulation:

    1.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    2.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Collagen II Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-2000 1:100-500 1:100-500

Product Details

Description

Collagen II Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen II.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human Predicted Reactivity: Mouse,Rat,Rabbit,Chicken,Pig,Cow,Dog,Guinea Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 120-230 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 117 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human Collagen II 1201-1300/1487

Sensitivity

Endogenous

Purification

Affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103040

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    2.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Collagen II is the major collagen synthesized by chondrocytes and a core structural component of articular cartilage, vitreous humour, inner ear, and nucleus pulposus[1]. Mechanistically, COL2A1 suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction and regulating BMP-SMAD1 signaling[2]. In disease models, COL2A1 loss accelerates chondrocyte hypertrophy, while degenerative human articular cartilage shows reduced COL2A1 with increased hypertrophic markers and BMP-SMAD1 activity[2]. Type II collagen degradation can also expose a cryptic peptide sequence that induces collagenase activity, MMP-13, and chondrocyte hypertrophy in articular cartilage explants[3]. Compared with type X collagen, which marks hypertrophic chondrocytes in osteoarthritis, collagen II better reflects the stable hyaline cartilage phenotype[4]. Compared with collagen I devices, collagen II biomaterials more directly match cartilage extracellular matrix composition and support cartilage engineering research[5]. For experimental applications, collagen II scaffolds help maintain or rejuvenate hyaline chondrocyte phenotype through Wnt-related mechanisms, supporting use in cartilage tissue engineering models[6].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:Isomer 2 is highly expressed in juvenile chondrocytes and lowly expressed in fetal chondrocytes.

  • Isoforms & Post-Translational Modification

    P02458 has 3 isomers: P02458-2: 141785 Da (predicted); P02458-1: 134389 Da (predicted); P02458-3: 29781 Da (predicted).
    The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2;Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains;Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline;Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains;O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide

  • Subunit

    Homotrimers of alpha 1(II) chains

  • SwissProt ID

    P02458

  • Gene ID
    1280 [NCBI]
  • Synonyms

    Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; Alpha-1 type II collagen; CO2A1_HUMAN.; CollagenII;

References

[1]. Norhalifah HK, et al. The genetic history of Peninsular Malaysia. Gene. 2016 Jul 15;586(1):129-35. [Content Brief]

[2]. Lian C, et al. Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction. Bone Res. 2019 Mar 6;7:8. [Content Brief]

[3]. van der Kraan PM, et al. Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration? Osteoarthritis Cartilage. 2012 Mar;20(3):223-32. [Content Brief]

[4]. Wu Z, et al. Collagen type II: From biosynthesis to advanced biomaterials for cartilage engineering. Biomater Biosyst. 2021 Nov 22;4:100030. [Content Brief]

[5]. Zhou H, et al. Rejuvenating Hyaline Cartilaginous Phenotype of Dedifferentiated Chondrocytes in Collagen II Scaffolds: A Mechanism Study Using Chondrocyte Membrane Nanoaggregates as Antagonists. ACS Nano. 2024 Jan 23;18(3):2077-2090. [Content Brief]

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Collagen II Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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