MEK2 Antibody (YA4966)

Cat. No.: HY-P85274: Data Sheet User Guide for Antibodies
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MEK2 Antibody (YA4966) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MEK2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MEK2 Antibody (YA4966) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MEK2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 46 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P36507

Gene ID

5605 [NCBI]

Immunogen

Recombinant human MEK2 Protein. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200-1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126)

Subcellular Localization：Cytoplasm; Membrane; Peripheral membrane protein

Subunit：Interacts with MORG1 (By similarity). Interacts with SGK1 (PubMed:19447520). Interacts with KSR1 (PubMed:10409742). Interacts with KSR1 and BRAF; the interaction with KSR1 mediates KSR1-BRAF dimerization (PubMed:29433126). Interacts with GLS (PubMed:22538822)

Synonyms

MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2

Documentation

User Guide for Antibodies (1077 KB)