Copy Link Print Information

Talin 1 Antibody (YA6816)

(Synonyms: ILWEQ antibody; Talin 1 antibody; Talin antibody; Talin-1 antibody; TLN 1 antibody; TLN antibody; Tln1 antibody; TLN1_HUMAN antibody; )
Customer Review

Based on 1 Customer Validation

Talin 1 Antibody (YA6816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Talin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Talin 1 Antibody (YA6816) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:2000

Product Details

Description

Talin 1 Antibody (YA6816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Talin 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 270 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 270 kDa
Immunogen

Recombinant protein within human Talin 1 aa 1,700-2,000 / 2,541.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719266

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Talin 1 Antibody (YA6816)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Talin 1 Antibody (HY-P87123) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), Raw264.7 (lane 4), HT-1080 (lane 5), and THP-1 (lane 6) using Talin 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system at a voltage of 100 V for 90 mins. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Talin 1 is a High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims

  • Subcellular Localization

    Cell projection, ruffle membrane,Cytoplasm, cytoskeleton,Cell surface,Cell junction, focal adhesion

  • Expression


    Tissue_Specificity: Expressed at low to non-detectable levels in many tissues but highly expressed in skin and pancreas with other tissues including kidney cortex, endocervix, testis, pituitary, liver, and spleen also showing robust expression
    Induction: By a combination of TGFB and EGF

  • Isoforms & Post-Translational Modification

    Q9Y490 has two isomers: Q9Y490-1: 269767 Da (predicted); Q9Y490-2: 271420 Da (predicted).

  • Subunit

    Part of a complex composed of THSD1, PTK2/FAK1, TLN1 and VCL (PubMed:29069646)

  • SwissProt ID

    Q9Y490

  • Gene ID
    7094 [NCBI]
  • Synonyms

    ILWEQ antibody; Talin 1 antibody; Talin antibody; Talin-1 antibody; TLN 1 antibody; TLN antibody; Tln1 antibody; TLN1_HUMAN antibody;

Talin 1 Antibody (YA6816) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Talin 1 Antibody (HY-P87123) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), Raw264.7 (lane 4), HT-1080 (lane 5), and THP-1 (lane 6) using Talin 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system at a voltage of 100 V for 90 mins. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Talin 1 Antibody (YA6816) Product Image
    1/16
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Talin 1 Antibody (YA6816) Product Image
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Talin 1 Antibody (HY-P87123) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), Raw264.7 (lane 4), HT-1080 (lane 5), and THP-1 (lane 6) using Talin 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system at a voltage of 100 V for 90 mins. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Experimental Validation Results for Talin 1 Antibody (YA6816)
  • Talin 1 Antibody (YA6816) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Talin 1 Antibody (HY-P87123) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Talin 1 Antibody (YA6816) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), Raw264.7 (lane 4), HT-1080 (lane 5), and THP-1 (lane 6) using Talin 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system at a voltage of 100 V for 90 mins. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Talin 1 Antibody (YA6816) Product Image WB
  • Talin 1 Antibody (YA6816) Product Image WB