2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Cystathionase Antibody (YA2452)

Cystathionase Antibody (YA2452)

Cat. No.: HY-P82707
COA
SDS
User Guide for Antibodies Technical Support

Cystathionase Antibody (YA2452) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cystathionase.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Documentation

Description

Cystathionase Antibody (YA2452) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cystathionase.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 45 kDa;
Observed band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Cystathionase aa1-55/405.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293T(lane 3(20ug) and K562(lane 4(20ug) using Cystathionase Antibody(HY-P82707) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Catalyzes the last step in the trans-sulfuration pathway from L-methionine to L-cysteine in a pyridoxal-5'-phosphate (PLP)-dependent manner, which consists on cleaving the L,L-cystathionine molecule into L-cysteine, ammonia and 2-oxobutanoate (PubMed:10212249, PubMed:18476726, PubMed:19261609, PubMed:19961860). Part of the L-cysteine derived from the trans-sulfuration pathway is utilized for biosynthesis of the ubiquitous antioxidant glutathione (PubMed:18476726). Besides its role in the conversion of L-cystathionine into L-cysteine, it utilizes L-cysteine and L-homocysteine as substrates (at much lower rates than L,L-cystathionine) to produce the endogenous gaseous signaling molecule hydrogen sulfide (H2S) (PubMed:10212249, PubMed:19019829, PubMed:19261609, PubMed:19961860). In vitro, it converts two L-cysteine molecules into lanthionine and H2S, also two L-homocysteine molecules to homolanthionine and H2S, which can be particularly relevant under conditions of severe hyperhomocysteinemia (which is a risk factor for cardiovascular disease, diabetes, and Alzheimer's disease) (PubMed:19261609). Lanthionine and homolanthionine are structural homologs of L,L-cystathionine that differ by the absence or presence of an extra methylene group, respectively (PubMed:19261609). Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function (PubMed:22169477). By generating the gasotransmitter H2S, it participates in a number of physiological processes such as vasodilation, bone protection, and inflammation (Probable) (PubMed:29254196). Plays an essential role in myogenesis by contributing to the biogenesis of H2S in skeletal muscle tissue (By similarity). Can also accept homoserine as substrate (By similarity). Catalyzes the elimination of selenocystathionine (which can be derived from the diet) to yield selenocysteine, ammonia and 2-oxobutanoate (By similarity)
Subcellular Localization：Cytoplasm
Expression：
Tissue_specificity:Highly expressed in liver (PubMed:10727430, PubMed:20305127) . Also in muscle and lower expression in most tissues except heart, pituitary gland, spleen, thymus, and vascular tissue, where it is hardly detected (PubMed:20305127)

Induction:Estrogen receptor alpha (ESR1) regulates CSE promoter activity and induces protein expression in human osteoblasts
Isoforms & Post-Translational Modification：P32929 has 3 isomers: P32929-1: 44508 Da (predicted); P32929-2: 39505 Da (predicted); P32929-3: 41260 Da (predicted).
Subunit：Homotetramer (PubMed:19019829).
RRID
Database

Entrez Gene: 1491 Human

SwissProt: P32929 Human

OMIM: 219500 Human

Research Field

Signal Transduction
Synonyms
CTH; Cystathionine gamma lyase; Cysteine desulfhydrase; Gamma cystathionase; Homoserine deaminase
Documentation
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Cystathionase Antibody (YA2452) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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